Human/Mouse/Rat GSK-3 beta Alexa Fluor® 594-conjugated Antibody

IC2506T Datasheet / COA / SDS

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IC2506T-100UG

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Human/Mouse/Rat GSK-3 beta Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat GSK-3 beta in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha.

Source

Monoclonal Rat IgG2b Clone # 272536

Immunogen

E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells

HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name

Glycogen Synthase Kinase 3 beta

Entrez Gene IDs

2932 (Human); 56637 (Mouse); 84027 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform