Human/Mouse/Rat GSK-3 beta Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2506N-100UG
Product Details
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Human/Mouse/Rat GSK-3 beta Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha.
Source
Monoclonal Rat IgG2b Clone # 272536
Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name
Glycogen Synthase Kinase 3 beta
Entrez Gene IDs
2932 (Human); 56637 (Mouse); 84027 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Product Datasheets

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