Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

Catalog # Availability Size / Price Qty
AF1935
AF1935-SP
Detection of Human HIF‑1 alpha /HIF1A by Western Blot.
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Product Details
Citations (47)
FAQs
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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat HIF-1 alpha /HIF1A.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HIF-1 alpha /HIF1A
Arg575-Asn826
Accession # Q16665.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunoprecipitation
2 µg/500 µg cell lysate
MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
0.3-15 µg/mL
See below
Knockout Validated
HIF-1 alpha /HIF1A is specifically detected in HeLa human cervical epithelial carcinoma cell line but is not detectable in HIF-1 alpha/HIF1A knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HIF-1a/HIF1A antibody by Western Blot. View Larger

Detection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-1a/HIF1A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of HIF-1a/HIF1A-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of HIF‑1 alpha /HIF1A-regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 µM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF-1a/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.

Immunocytochemistry HIF-1a/HIF1A Antibody Specificity is Shown by Immunocytochemistry in Knockout Cell Line by Immunocytochemistry (ICC). View Larger

HIF‑1 alpha /HIF1A Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF-1a/HIF1A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF-1a/HIF1A knockout (KO) HeLa Human Cell Line cell line using Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human HIF-1a/HIF1A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human HIF‑1 alpha /HIF1A by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF-1a/HIF1A at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Mouse HIF-1 alpha by Simple Western View Larger

Detection of Mouse HIF-1 alpha by Simple Western Ten days of IH increases hippocampal HIF1a and disrupts Barnes maze performance in wild-type mice but not in HIF1a+/−. A, left, Representative digitized Western blotting images for HIF1a (103 kDa) and PCNA (40 kDa) in hippocampal nuclear protein fractions from control (n = 4) and IH10 (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a was increased in IH10 when compared with control (p = 0.019). B, Total latency to exit the Barnes maze during three training sessions in control (n = 10) and in IH10 (n = 11). Each blue (control) and red (IH10) line represents an individual performance during training. Training to the exit was conducted over three sessions. Each session was separated by 24 hours. C, Left, During the probe trial, the distance traveled to initially enter the exit zone was shorter in control when compared with IH10 (p = 0.048). Right, Latency to initial entry was smaller in control as well (p = 0.034). D, Heat maps of the mean entry probability across all false exits (1–19) and the exit zone during probe trial for the control and IH10. Comparison of entry probability into the exit zone during the probe trial reveals that control has a greater probability for entering the exit zone when compared with IH10 (p = 0.004). E, Left, Representative digitized Western blotting images HIF1a and PCNA in hippocampal nuclear protein fractions from 0-HIF1a+/− (n = 4) and 10-HIF1a+/− (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a is similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.84). F, Total latency to exit the Barnes maze during three training sessions in 0-HIF1a+/− (n = 7) and in 10-HIF1a+/− (n = 8). Each gray (0-HIF1a+/−) and yellow (10-HIF1a+/−) line represents an individual performance during training. All experimental groups exhibit decreased total latency over the course of training. G, Left, In HIF1a+/−, the distance initial to initial entry into the exit zone was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.55). Right, Latency to initial entry into the exit zone during the probe trial were similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.39). H, Heat maps of the mean entry probability into all zones during the probe trial for 0-HIF1a+/− and 10-HIF1a+/−. Entry probability was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.21); *p < 0.05; **p < 0.01; N.S., p > 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32493757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human HIF-1 alpha by Western Blot View Larger

Detection of Human HIF-1 alpha by Western Blot The DU145FP cells express higher levels of cancer stemness related genes. (A) Transcriptome analyses of prostate cancer stemness-related markers. The Log2 transformed ratio of FPKM values (e.g., FP/WT) are indicated by color-coded index bars. (B) Cells were stained with fluorophore conjugated monoclonal antibodies against CD44 and EPCAM, the antibody against integrin alpha 2 beta 3 without fluorophore conjugation was detected by using secondary antibody conjugated with fluorophore, and stained cells were analyzed by flow cytometry. The scatter plots and histograms show representative results. CTRL denote cell stained with isotype control antibody or only secondary antibody; ST denote cell stained with antibody against EPCAM and integrin alpha 2 beta 3; US denote unstained cells. (C) Immunoblotting confirmation of the protein expression of A-tubulin, GAPDH, SOX9, SLUG, HIF1A, HIF2A, CD44 and integrin alpha 2 beta 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28698547), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western Detection of Mouse HIF-1 alpha by Simple Western View Larger

Detection of Mouse HIF-1 alpha by Simple Western Ten days of IH increases hippocampal HIF1a and disrupts Barnes maze performance in wild-type mice but not in HIF1a+/−. A, left, Representative digitized Western blotting images for HIF1a (103 kDa) and PCNA (40 kDa) in hippocampal nuclear protein fractions from control (n = 4) and IH10 (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a was increased in IH10 when compared with control (p = 0.019). B, Total latency to exit the Barnes maze during three training sessions in control (n = 10) and in IH10 (n = 11). Each blue (control) and red (IH10) line represents an individual performance during training. Training to the exit was conducted over three sessions. Each session was separated by 24 hours. C, Left, During the probe trial, the distance traveled to initially enter the exit zone was shorter in control when compared with IH10 (p = 0.048). Right, Latency to initial entry was smaller in control as well (p = 0.034). D, Heat maps of the mean entry probability across all false exits (1–19) and the exit zone during probe trial for the control and IH10. Comparison of entry probability into the exit zone during the probe trial reveals that control has a greater probability for entering the exit zone when compared with IH10 (p = 0.004). E, Left, Representative digitized Western blotting images HIF1a and PCNA in hippocampal nuclear protein fractions from 0-HIF1a+/− (n = 4) and 10-HIF1a+/− (n = 4). Right, Quantification of HIF1a protein normalized to PCNA revealed that nuclear HIF1a is similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.84). F, Total latency to exit the Barnes maze during three training sessions in 0-HIF1a+/− (n = 7) and in 10-HIF1a+/− (n = 8). Each gray (0-HIF1a+/−) and yellow (10-HIF1a+/−) line represents an individual performance during training. All experimental groups exhibit decreased total latency over the course of training. G, Left, In HIF1a+/−, the distance initial to initial entry into the exit zone was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.55). Right, Latency to initial entry into the exit zone during the probe trial were similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.39). H, Heat maps of the mean entry probability into all zones during the probe trial for 0-HIF1a+/− and 10-HIF1a+/−. Entry probability was similar between 0-HIF1a+/− and 10-HIF1a+/− (p = 0.21); *p < 0.05; **p < 0.01; N.S., p > 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32493757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. HIF-1alpha regulates CD55 expression in airway epithelium.
    Authors: Pandya PH, Wilkes DS.
    Am J Respir Cell Mol Biol
  2. Local lung hypoxia determines epithelial fate decisions during alveolar regeneration
    Authors: Ying Xi, Thomas Kim, Alexis N. Brumwell, Ian H. Driver, Ying Wei, Victor Tan et al.
    Nature Cell Biology
  3. MTA1 aggravates experimental colitis in mice by promoting transcription factor HIF1A and up-regulating AQP4 expression
    Authors: Ping Li, Dong-Ping Shi, Tao Jin, Dong Tang, Wei Wang, Liu-Hua Wang
    Cell Death Discovery
  4. LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-kappa B pathways under hypoxic-ischemic conditions
    Authors: Yoshinori Aoki, Hongmei Dai, Fumika Furuta, Tomohisa Akamatsu, Takuya Oshima, Naoto Takahashi et al.
    Cell Communication and Signaling
  5. Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer
    Authors: Becker LM, O'Connell JT, Vo AP et al.
    Cell Rep
  6. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells
    Authors: Ingrid Espinoza, Marcelo J. Sakiyama, Tangeng Ma, Logan Fair, Xinchun Zhou, Mohamed Hassan et al.
    Frontiers in Oncology
  7. HRAS as a potential therapeutic target of salirasib RAS inhibitor in bladder cancer
    Authors: Satoshi Sugita, Hideki Enokida, Hirofumi Yoshino, Kazutaka Miyamoto, Masaya Yonemori, Takashi Sakaguchi et al.
    International Journal of Oncology
  8. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism
    Authors: Ho JJD, Balukoff NC, Theodoridis PR et al.
    Nat Commun
  9. Human TM9SF4 Is a New Gene Down-Regulated by Hypoxia and Involved in Cell Adhesion of Leukemic Cells
    Authors: Rosa Paolillo, Isabella Spinello, Maria Teresa Quaranta, Luca Pasquini, Elvira Pelosi, Francesco Lo Coco et al.
    PLOS ONE
  10. Hypoxia-induced HIF1 alpha targets in melanocytes reveal a molecular profile associated with poor melanoma prognosis
    Authors: Stacie K. Loftus, Laura L. Baxter, Julia C. Cronin, Temesgen D. Fufa, William J. NISC Comparative Sequencing Program, William J. NISC Comparative Sequencing Program
    Pigment Cell & Melanoma Research
  11. Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient Survival
    Authors: Eleanor R. Burgess, Rebekah L. I. Crake, Elisabeth Phillips, Helen R. Morrin, Janice A. Royds, Tania L. Slatter et al.
    Frontiers in Oncology
  12. A HIF1a-Dependent Pro-Oxidant State Disrupts Synaptic Plasticity and Impairs Spatial Memory in Response to Intermittent Hypoxia.
    Authors: Arias-Cavieres A, Khuu M A et al.
    Eneuro
    Species: Mouse
    Sample Types:
    Applications: Simple Western
  13. Lactate limits CNS autoimmunity by stabilizing HIF-1? in dendritic cells
    Authors: Sanmarco, LM;Rone, JM;Polonio, CM;Fernandez Lahore, G;Giovannoni, F;Ferrara, K;Gutierrez-Vazquez, C;Li, N;Sokolovska, A;Plasencia, A;Faust Akl, C;Nanda, P;Heck, ES;Li, Z;Lee, HG;Chao, CC;Rejano-Gordillo, CM;Fonseca-Castro, PH;Illouz, T;Linnerbauer, M;Kenison, JE;Barilla, RM;Farrenkopf, D;Stevens, NA;Piester, G;Chung, EN;Dailey, L;Kuchroo, VK;Hava, D;Wheeler, MA;Clish, C;Nowarski, R;Balsa, E;Lora, JM;Quintana, FJ;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  14. Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction
    Authors: Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA;
    Journal of the American Heart Association
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Engineered probiotics limit CNS autoimmunity by stabilizing HIF-1alpha in dendritic cells
    Authors: LM Sanmarco, JM Rone, CM Polonio, F Giovannoni, GF Lahore, K Ferrara, C Gutierrez-, N Li, A Sokolovska, A Plasencia, CF Akl, P Nanda, ES Heck, Z Li, HG Lee, CC Chao, CM Rejano-Gor, PH Fonseca-Ca, T Illouz, M Linnerbaue, JE Kenison, RM Barilla, D Farrenkopf, G Piester, L Dailey, VK Kuchroo, D Hava, MA Wheeler, C Clish, R Nowarski, E Balsa, JM Lora, FJ Quintana
    bioRxiv : the preprint server for biology, 2023-03-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Extreme Hypoxia Causing Brady-Arrythmias During Apnea in Elite Breath-Hold Divers
    Authors: T Kjeld, AB Isbrand, K Linnet, B Zerahn, J Højberg, EG Hansen, LC Gormsen, J Bejder, T Krag, J Vissing, HE Bøtker, HC Arendrup
    Frontiers in Physiology, 2021-12-03;12(0):712573.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. FLI1 mediates the selective expression of hypoxia-inducible factor-1 target genes in endothelial cells under hypoxic conditions
    Authors: G Zeng, T Wang, J Zhang, YJ Kang, L Feng
    FEBS Open Bio, 2021-06-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  18. LncRNA-SNHG6 promotes the progression of hepatocellular carcinoma by targeting miR-6509-5p and HIF1A
    Authors: X Fan, Z Zhao, J Song, D Zhang, F Wu, J Tu, M Xu, J Ji
    Cancer Cell International, 2021-03-04;21(1):150.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon
    Authors: B Di Marco, EE Crouch, B Shah, C Duman, MF Paredes, C Ruiz de Al, EJ Huang, J Alfonso
    Cell Rep, 2020-10-13;33(2):108256.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology
    Authors: M Felsenstei, A Blank, AD Bungert, A Mueller, A Ghori, I Kremenetsk, O Rung, T Broggini, K Turkowski, L Scherschin, J Raggatz, P Vajkoczy, S Brandenbur
    Cancers (Basel), 2020-07-13;12(7):.
    Species: Mouse
    Sample Types: Brain Section
    Applications: IHC-F
  21. The critical role of MMP14 in adipose tissue remodeling during obesity
    Authors: X Li, Y Zhao, C Chen, L Yang, HH Lee, Z Wang, N Zhang, MG Kolonin, Z An, X Ge, PE Scherer, K Sun
    Mol. Cell. Biol., 2020-03-30;0(0):.
    Species: Human
    Sample Types: Chromatin
    Applications: ChIP (chromatin immunoprecipit
  22. HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury
    Authors: AN Billin, SE Honeycutt, AV McDougal, JP Kerr, Z Chen, JM Freudenber, DK Rajpal, G Luo, HF Kramer, RS Geske, F Fang, B Yao, RV Clark, J Lepore, A Cobitz, R Miller, K Nosaka, AC Hinken, AJ Russell
    Skelet Muscle, 2018-11-13;8(1):35.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. Co-Expression of VEGF and IL-6 Family Cytokines is Associated with Decreased Survival in HER2 Negative Breast Cancer Patients: Subtype-Specific IL-6 Family Cytokine-Mediated VEGF Secretion
    Authors: K Tawara, H Scott, J Emathinger, A Ide, R Fox, D Greiner, D LaJoie, D Hedeen, M Nandakumar, AJ Oler, R Holzer, C Jorcyk
    Transl Oncol, 2018-11-12;12(2):245-255.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. PRKAA1/AMPK?1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis
    Authors: Q Yang, J Xu, Q Ma, Z Liu, V Sudhahar, Y Cao, L Wang, X Zeng, Y Zhou, M Zhang, Y Xu, Y Wang, NL Weintraub, C Zhang, T Fukai, C Wu, L Huang, Z Han, T Wang, DJ Fulton, M Hong, Y Huo
    Nat Commun, 2018-11-07;9(1):4667.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis
    Authors: Z Liu, S Yan, J Wang, Y Xu, Y Wang, S Zhang, X Xu, Q Yang, X Zeng, Y Zhou, X Gu, S Lu, Z Fu, DJ Fulton, NL Weintraub, RB Caldwell, W Zhang, C Wu, XL Liu, JF Chen, A Ahmad, I Kaddour-Dj, M Al-Shabraw, Q Li, X Jiang, Y Sun, A Sodhi, L Smith, M Hong, Y Huo
    Nat Commun, 2017-09-19;8(1):584.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
    Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
    J. Biol. Chem., 2017-07-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
    Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
    Sci Rep, 2017-07-11;7(1):5081.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Motor neurons control blood vessel patterning in the developing spinal cord
    Authors: P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al
    Nat Commun, 2017-03-06;8(0):14583.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1?
    Mol Cell Biol, 2016-07-14;0(0):.
    Species: Human
    Sample Types: Chromatin
    Applications: ChIP
  30. Identification of ?-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation
    PLoS ONE, 2016-05-25;11(5):e0156325.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice
    Authors: Gabi U Dachs
    Hypoxia (Auckl), 2016-04-08;4(0):41-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
    Authors: Zuo R, Gu X, Qi Q, Wang T, Zhao X, Liu J, Yang Z
    J Biol Chem, 2015-07-15;290(35):21280-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. High Glucose Up-regulates ADAM17 through HIF-1alpha in Mesangial Cells.
    Authors: Li R, Uttarwar L, Gao B, Charbonneau M, Shi Y, Chan J, Dubois C, Krepinsky J
    J Biol Chem, 2015-07-14;290(35):21603-14.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: ChIP
  34. Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression.
    Authors: Tholen M, Wolanski J, Stolze B, Chiabudini M, Gajda M, Bronsert P, Stickeler E, Rospert S, Reinheckel T
    J Biol Chem, 2015-05-08;290(25):15758-69.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis.
    Authors: Palladino M, Shah A, Tyson R, Horvath J, Dugan C, Karpodinis M
    Reprod Biol Endocrinol, 2012-12-05;10(0):104.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP, Direct ELISA
  36. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
    Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
    BMC Cancer, 2012-05-29;12(0):201.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia.
    Authors: Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G
    Cardiovasc. Res., 2011-07-19;92(2):247-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  38. Development of an in vitro model of myotube ischemia.
    Authors: Joshi D, Patel H, Baker DM, Shiwen X, Abraham DJ, Tsui JC
    Lab. Invest., 2011-05-23;91(8):1241-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  39. HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.
    Authors: Bollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP
    Cancer Res., 2010-08-24;70(20):7862-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
    Authors: Wang B, Wood IS, Trayhurn P
    J. Endocrinol., 2008-05-07;198(1):127-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
    Authors: Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N
    Blood, 2007-09-11;110(13):4464-75.
    Species: Human
    Sample Types: Nuclear Extract
    Applications: Western Blot
  42. Amylin deposition activates HIF1 alpha and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates
    Authors: Miao Liu, Nan Li, Chun Qu, Yilin Gao, Lijie Wu, Liangbiao George Hu
    Communications Biology
  43. Astrocyte HIF-2α supports learning in a passive avoidance paradigm under hypoxic stress
    Authors: Cindy V Leiton, Elyssa Chen, Alissa Cutrone, Kristy Conn, Kennelia Mellanson, Dania M Malik et al.
    Hypoxia (Auckl)
  44. Effects of lipopolysaccharide-induced inflammation on hypoxia and inflammatory gene expression pathways of the rat testis
    Authors: Michael A. Palladino, Genevieve A. Fasano, Dharm Patel, Christine Dugan, Marie London
    Basic and Clinical Andrology
  45. Galectin-1 suppression delineates a new strategy to inhibit myeloma-induced angiogenesis and tumoral growth in vivo
    Authors: P Storti, V Marchica, I Airoldi, G Donofrio, E Fiorini, V Ferri et al.
    Leukemia
  46. A translational program that suppresses metabolism to shield the genome
    Authors: NC Balukoff, JJD Ho, PR Theodoridi, M Wang, M Bokros, LM Llanio, JR Krieger, JH Schatz, S Lee
    Nat Commun, 2020-11-13;11(1):5755.
  47. New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma
    Authors: Ioana V. Militaru, Alina Adriana Rus, Cristian V.A. Munteanu, Georgiana Manica, Stefana M. Petrescu
    Frontiers in Oncology

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Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody
By Anonymous on 12/04/2019
Sample Tested: HASMC human aortic smooth muscle cells Species: Human

I have tried HIF1A antibodies from several companies, this one is good compared to others, but sometimes there are multiple bands. 1:500