Human/Mouse/Rat Histamine H3 R Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB102001-100
MAB102001-SP
Detection of HRH3  in HEK293 Human Cell Line Transfected with Human HRH3 and eGFP by Flow  Cytometry.
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Human/Mouse/Rat Histamine H3 R Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Histamine H3 R in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2420B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing Human/Mouse/Rat Histamine H3 R
Accession # Q9Y5N1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of HRH3  in HEK293 Human Cell Line Transfected with Human HRH3 and eGFP by Flow  Cytometry. View Larger

Detection of HRH3 in HEK293 Human Cell Line Transfected with Human HRH3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human HRH3 or (B) irrelevant protein, and EGFP, was stained with Rabbit Anti-Human HRH3 Monoclonal Antibody (Catalog # MAB102001) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG isotype control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine H3 R

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors Histamine H1 R, Histamine H2 R, Histamine H3 R and Histamine H4 R. HRH3 encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. Histamine H3 R is an integral membrane protein and can regulate neurotransmitter release. It can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.

Long Name
Histamine H3 Receptor
Entrez Gene IDs
11255 (Human); 99296 (Mouse)
Alternate Names
GPCR97; GPCR97G protein-coupled receptor 97; G-protein coupled receptor 97; H3R; HH3R; Histamine H3 R; histamine H3 receptor; Histamine H3R; histamine receptor H3; HRH3

Product Datasheets

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