Human/Mouse/Rat IKK gamma Antibody

Name: Human/Mouse/Rat IKK gamma Antibody
Brand: R&D Systems
SKU: AF4365
Price: 389 USD
Availability: InStock

R&D Systems | Catalog # AF4365

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all IKK gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IKK gamma
Met1-Glu411
Accession # O88522

Specificity

Detects endogenous human, mouse, and rat IKK gamma in Western blot.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat IKK gamma Antibody

Detection of Mouse and Rat IKK gamma by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4365) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for IKK gamma at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat IKK gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblasts embryonic fibroblast, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IKK gamma

I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.