Human/Mouse/Rat N-Cadherin Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: N-Cadherin

N-Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130-135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N-Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN-Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15-20 kDa prodomain (aa 26-159) is cleaved by proprotein convertase, and the mature molecule (aa 160-906) is transported to the surface. Mature N-Cadherin contains a 565 aa extracellular region (aa 160-724) that possesses five cadherin domains (aa 160-714), and a 161 aa cytoplasmic tail that undergoes phosphorylation at Tyr785. There is one splice variant that contains a 10 aa substitution for aa 839-906. Over aa 160-724, human N Cadherin shares 98% aa identity with mouse N-Cadherin.

Product Datasheets

Related Research Areas

• Adhesion Molecules
• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Cadherin Superfamily
• Early Mesodermal Lineage Markers
• Mesenchymal Cells and EMT
• Mesenchymal Stem Cell Markers
• Neural Stem Cell Markers
• Synaptic Adhesion Molecules
• Synaptic Proteins