|Detection of Human, Mouse, and Rat N‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for N‑Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of N‑Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426, filled histogram) or Sheep IgG control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).|
|N‑Cadherin in A549 Human Cell Line. N‑Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
|N‑Cadherin in Human Brain. N‑Cadherin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Mouse/Rat N‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6426) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
N-Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130-135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N-Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN-Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15-20 kDa prodomain (aa 26-159) is cleaved by proprotein convertase, and the mature molecule (aa 160-906) is transported to the surface. Mature N-Cadherin contains a 565 aa extracellular region (aa 160-724) that possesses five cadherin domains (aa 160-714), and a 161 aa cytoplasmic tail that undergoes phosphorylation at Tyr785. There is one splice variant that contains a 10 aa substitution for aa 839-906. Over aa 160-724, human N Cadherin shares 98% aa identity with mouse N-Cadherin.
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