Human/Mouse/Rat p27/Kip1 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human p27/Kip1 in direct ELISAs. Specificity was demonstrated by comparing staining on tissue sections of normal versus p27/Kip1 knockout mice.
  • Source
    Monoclonal Rat IgG2A Clone # 341116
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human p27/Kip1
    Met1-Thr198
    Accession # P46527
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
p27/Kip1 in Human Prostate Cancer Tissue.

p27/Kip1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Rat Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB22561) overnight at 4 °C. Tissue was stained with the Human p27 Immunohistochemistry Detection Kit (brown; Catalog # CTS2256) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Human p27/Kip1 shares 88% and 87% amino acid sequence identity with mouse and rat p27/Kip1, respectively, while mouse and rat p27/Kip1 share 96% amino acid sequence identity.

  • Long Name:
    p27 Cyclin Dependent Kinase 4 Inhibitor 1B
  • Entrez Gene IDs:
    1027 (Human); 12576 (Mouse); 83571 (Rat)
  • Alternate Names:
    CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1
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