Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 341116
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p27/Kip1
Met1-Thr198
Accession # P46527

Specificity

Detects human p27/Kip1 in direct ELISAs. Specificity was demonstrated by comparing staining on tissue sections of normal versus p27/Kip1 knockout mice.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat p27/Kip1 Antibody

p27/Kip1 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

p27/Kip1 in Human Prostate Cancer Tissue.

p27/Kip1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Rat Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB22561) overnight at 4 °C. Tissue was stained with the Human p27 Immunohistochemistry Detection Kit (brown; Catalog # CTS2256) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse/Rat p27/Kip1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB22561 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Human p27/Kip1 shares 88% and 87% amino acid sequence identity with mouse and rat p27/Kip1, respectively, while mouse and rat p27/Kip1 share 96% amino acid sequence identity.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Gene Symbol

CDKN1B

UniProt

P46527

Additional p27/Kip1 Products

Product Documents for Human/Mouse/Rat p27/Kip1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p27/Kip1 Antibody

For research use only

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Customer Reviews for Human/Mouse/Rat p27/Kip1 Antibody (1)

5 out of 5
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  • Human/Mouse/Rat p27/Kip1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer tissue
    Species: Mouse
    Verified Customer | Posted 01/31/2022
    Human/Mouse/Rat p27/Kip1 Antibody MAB22561

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail