Human/Mouse/Rat Parvalbumin  alpha Antibody

Catalog # Availability Size / Price Qty
AF5058
AF5058-SP
Human/Mouse/Rat Parvalbumin  alpha  Antibody in Data
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat Parvalbumin  alpha Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Parvalbumin  alpha in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Parvalbumin alpha
Ser2-Ser110
Accession # P20472
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat Parvalbumin a antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Parvalbumin alpha by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Parvalbumin a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Parvalbumin a antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Parvalbumin alpha in Human Brain. Parvalbumin a was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 5 µg/mL Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Rat Parvalbumin a antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat Parvalbumin alpha by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 15 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human and Mouse Parvalbumin a antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse Parvalbumin alpha by Simple WesternTM. Simple Western lane view shows lysates of mouse brain (cortex) and human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 14 and 17 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Parvalbumin alpha

Parvalbumin (Parvalbumin alpha ) is a 12 kDa member of the parvalbumin family of Ca++-binding proteins. In human, it is expressed in intrafusal muscle fibers, plus GABAergic interneurons and cerebellar Purkinje and basket cells. It presumably acts as a Ca++ buffer that shortens the duration of fiber contraction. Human Parvalbumin is 110 amino acids (aa) in length. It contains two EF-hand domains (aa 39-74 and 78-110) that bind calcium. There are three potential isoform variants. One shows an alternate start site at Met33, a second shows a six aa substitution for the C-terminal nine amino acids and a third shows a deletion of Gly99-Val100. Human Parvalbumin  alpha is 51% aa identical to human Parvalbumin beta and is 87% plus 92% aa identical to mouse and rat Parvalbumin, respectively.

Entrez Gene IDs
5816 (Human); 19293 (Mouse); 25269 (Rat)
Alternate Names
Parvalbumin alpha; PVALB

Product Datasheets

Citations for Human/Mouse/Rat Parvalbumin  alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018;8(1):603.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Nogo receptor 1 is expressed by nearly all retinal ganglion cellsPV expression in Figure 3
    Authors: AM Solomon, T Westbrook, GD Field, AW McGee
    PLoS ONE, 2018;13(5):e0196565.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer
    Authors: C Totland, T Kråkenes, K Mazengia, M Haugen, C Vedeler
    Oncotarget, 2018;9(35):23975-23986.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Nogo Receptor 1 Confines a Disinhibitory Microcircuit to the Critical Period in Visual Cortex
    Authors: Aaron W McGee
    J. Neurosci., 2016;36(43):11006-11012.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Neuronal cell type-specific alternative splicing is regulated by the KH domain protein SLM1.
    Authors: Iijima, Takatosh, Iijima, Yoko, Witte, Harald, Scheiffele, Peter
    J Cell Biol, 2014;204(3):331-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Plasticity of binocularity and visual acuity are differentially limited by nogo receptor.
    Authors: Stephany C, Chan L, Parivash S, Dorton H, Piechowicz M, Qiu S, McGee A
    J Neurosci, 2014;34(35):11631-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Parvalbumin-containing chandelier and basket cell boutons have distinctive modes of maturation in monkey prefrontal cortex.
    Authors: Fish K, Hoftman G, Sheikh W, Kitchens M, Lewis D
    J Neurosci, 2013;33(19):8352-8.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human/Mouse/Rat Parvalbumin  alpha Antibody

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Human/Mouse/Rat Parvalbumin  alpha Antibody
By Anonymous on 07/25/2019
Application: ICC/IF Sample Tested: Adult brain,Adult brain (hippocampus) Species: Mouse