Human/Mouse/Rat Parvalbumin  alpha Antibody

Detection of Human and Mouse Parvalbumin alpha by Simple Western^TM.

Simple Western lane view shows lysates of mouse brain (cortex) and human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for Parvalbumin a at approximately 14 and 17 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Parvalbumin a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5058) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Parvalbumin alpha by Simple Western

Altered NARP expression impacts the expression of parvalbumin in inhibitory neurons in socially isolated mice. (A) Schematic diagram of molecular & behavioral analyses. Molecular analysis was conducted at P35 & adulthood, & behavioral analysis was conducted in adulthood. (B) NARP mRNA levels in the medial PFC of jSI mice were lower than those in GH mice at P35, but not at adult [2-way ANOVA, age (P35 & adult) x housing (GH & jSI) interaction F(1. 24) = 6.677 p = 0.0163, age F(1. 24) = 3.505 p = 0.0734, housing F(1. 24) = 4.600 p = 0.0423; Tukey test p = 0.0135 (GH vs jSI at P35), n = 7 (GH, P35), n = 7 (jSI, P35), n = 7 (GH, adult), n = 7 (jSI, adult)]. (C) NARP protein levels in the medial PFC of jSI mice were lower than those in GH mice at P35 (two-tailed t-test, t12 = 2.348, p = 0.0369). (D) Representative images of NARP protein expression in the medial PFC of GH & jSI mice. (E) PV mRNA levels in the medial PFC of jSI mice were lower than those in GH mice at adult, but not at P35 [2-way ANOVA, age (P35 & adult) x housing (GH & jSI) interaction F(1. 24) = 0.4791, p = 0.4955; age F(1. 24) = 3.821, p = 0.0624; housing F(1. 24) = 11.71, p = 0.0022; Tukey test p = 0.0361 (GH vs jSI at adult), n = 7 (GH, P35), n = 7 (jSI, P35), n = 7 (GH, adult), n = 7 (jSI, adult)]. (F) PV protein levels in the medial PFC of jSI mice were lower than those in GH mice at adult (two-tailed t-test, t12 = 3.440, p = 0.0049). (G) Representative images of PV protein expression in the medial PFC of GH & jSI mice. NARP, Neuronal Activity-Regulated Pentraxin; PV, Parvalbumin; PFC, Prefrontal Cortex; GH, Group-Housed; jSI, Juvenile Social Isolation; P35, Postnatal Day 35; Adult/Adulthood, Postnatal Day 63–70; ANOVA, Analysis of Variance. * 0.01 ≤ p < 0.05; ** 0.001 ≤ p < 0.01. Image collected & cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fpsyt.2024.1403476/full), licensed under a CC-BY license. Not internally tested by R&D Systems.