Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antibody

AF2506 has been discontinued. View all MEK1/MEK2 products.
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat MEK1 and MEK2 dually phosphorylated at S218/S222 and S222/S226, respectively.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human, mouse, and rat MEK1 S218/S222 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed human peripheral blood mononuclear cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho‑MEK1 (S218/S222) and MEK2 (S222/S226) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-MEK1 (S218/S222)/MEK2 (S222/S226) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2506), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MEK1 (S218/S222) and MEK2 (S222/S226) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks, including the Raf kinases. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

  • Long Name:
    Mitogen-activated Protein Kinase Kinase 1
  • Alternate Names:
    MEK1/MEK2
Related Research Areas

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