Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA

Catalog # Availability Size / Price Qty
DB100C
PDB100C
Human TGF-beta 1 Cell culture supernate/Urine Standard Curve
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Product Details
Citations (3)
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Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Summary

Assay Length
4.5 hours
Specificity
Natural and recombinant TGF-beta 1. This assay also recognizes human TGF-beta 1.2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in cell culture supernates, serum, platelet-poor plasma, and urine. It contains CHO cell-expressed recombinant TGF-beta 1 and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural TGF-beta 1.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, Platelet-poor Plasma, Platelet-poor EDTA Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 4 5 6 1 2 3 4 5 6
n 20 20 20 20 20 20 20 20 20 20 20 20
Mean 317 683 1271 309 708 1072 312 657 1184 303 622 1092
Standard Deviation 11.3 42.2 31.1 15.3 40 44.3 26.1 46.6 92.3 21.2 47.2 70
CV% 3.6 6.2 2.4 5 5.6 4.1 8.4 7.1 7.8 7 7.6 6.4

Recovery

The recovery of TGF-beta 1 spiked to levels throughout the range of the assay in activated samples was evaluated.

Sample Type Average % Recovery Range %
Media + FBS (n=4) 103 80-125
Platelet-poor EDTA Plasma (n=4) 90 83-98
Platelet-poor Plasma (n=4) 90 77-107
Serum-free Cell Culture Media (n=4) 87 82-95
Urine (n=4) 114 105-123

Linearity

To assess linearity of the assay, the activated samples containing and/or spiked with high concentrations of TGF-beta 1, were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Data Examples

Human TGF-beta 1 Cell culture supernate/Urine Standard Curve

Human TGF-beta 1 Serum/Platelet-poor Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Citations for Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration
    Authors: KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Development of Injectable Polydactyly-Derived Chondrocyte Sheets
    Authors: S Wasai, E Toyoda, T Takahashi, M Maehara, E Okada, R Uchiyama, T Akamatsu, M Watanabe, M Sato
    International Journal of Molecular Sciences, 2021;22(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021;22(6):.
    Species: Human
    Sample Types: Plasma

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