Human/Mouse/Rat Syntaxin 7 Antibody

Catalog # Availability Size / Price Qty
AF5478
AF5478-SP
Detection of Human Syntaxin 7 by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Syntaxin 7 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Syntaxin 7 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Syntaxin 7
Asn21-Glu187
Accession # O15400
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Syntaxin 7 antibody by Western Blot. View Larger

Detection of Human Syntaxin 7 by Western Blot. Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human, Mouse, and Rat Syntaxin 7 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Syntaxin 7 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, BaF3 mouse pro-B cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Syntaxin 7 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Syntaxin 7 in HeLa Human Cell Line. Syntaxin 7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Detection of Syntaxin 7 in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells. Syntaxin 7 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin 7

Syntaxin 7 (STX7) is a widely expressed protein embedded in endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX7 is involved in endocytic trafficking from early endosomes to late endosomes and lysosomes. This is in contrast to STX8, which is involved in clathrin-independent vesicular transport. Human STX7 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 261 amino acids (aa) in length. It contains a coiled-coil region (aa 47‑69), a t-SNARE domain (aa 165‑227) that is likely involved in protein-protein interactions, and a short, two amino acid, C-terminal luminal sequence. Over aa 21‑187, human STX7 shares 97% aa identity with mouse STX7.

Entrez Gene IDs
8417 (Human); 53331 (Mouse); 60466 (Rat)
Alternate Names
STX7; Syntaxin 7; syntaxin-7

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Citation for Human/Mouse/Rat Syntaxin 7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles.
    Authors: Sang Su Woo, Declan J James, Thomas F J Martin
    Molecular Biology of the Cell, 2017;0(0):1939-4586.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Syntaxin 7 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human/Mouse/Rat Syntaxin 7 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Syntaxin 7 Antibody
By Anonymous on 11/09/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human