Human/Mouse/Rat TrkB Antibody

Catalog # Availability Size / Price Qty
MAB1494
MAB1494-SP
Human/Mouse/Rat TrkB Antibody in Western Blot
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Human/Mouse/Rat TrkB Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TrkA, recombinant rat TrkA, or recombinant mouse TrkC is observed.
Source
Monoclonal Rat IgG2B Clone # 225105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TrkB
Cys32-His429
Accession # P15209
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat TrkB by Western Blot. View Larger

Detection of Human, Mouse, and Rat TrkB by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, mouse brain (cortex) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 95 kDa and 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse TrkB by Western Blot. View Larger

Detection of Mouse TrkB by Western Blot. Western blot shows lysate of mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3 and NT-4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize with or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, human and mouse TrkB show 94% amino acid sequence identity (5‑6). The proteins also exhibit cross-species activity. The primary location of TrkB expression is in the central and peripheral nervous systems. Low level TrkB expression has also been observed in a wide variety of tissues outside the nervous system (6).

References
  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Nakagawara A. et al. (1995) Genomics. 25:538.
  6. Klein, R. et al. (1989) EMBO J. 8:3701.
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs
4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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