Detection of Human and Mouse RCOR1/CoREST by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse RCOR1/CoREST Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6047) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RCOR1/CoREST at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
RCOR1 (REST [RE-1 silencing transcription factor] CORepressor 1; also CoREST) is a 64‑66 kDa member of the CoREST family of proteins. It is a nuclear protein that is ubiquitously expressed. RCOR1 serves as a molecular bridge, linking REST with Sin3, HDAC, LSD1, histone H3 K9 methyltransferases, and MeCP2. This creates a complex that represses the expression of neuron‑specific genes in nonneuronal cells, principally by coordinating the demethylation of Lys4 in histone H3. Human RCOR1 is 482 amino acids (aa) in length. It contains one ELM2 domain (aa 102‑157) that interacts with HDAC, and two SANT sequences (aa 187‑238 and 378‑429) that bind DNA tandem repeats. There are three potential RCOR1 splice variants. One contains a four aa substitution for aa 296‑482, a second shows an alternative start site 3 aa upstream of the standard site, while a third shares the same upstream start site accompanied by a 15 aa substitution for aa 471‑482. Over aa 394‑482, human RCOR1 shares 93% aa identity with mouse RCOR1.
REST Corepressor 1
Entrez Gene IDs:
CoREST; KIAA0071CORESTRCORREST corepressor; Protein CoREST; RCOR1; RCOR1/CoREST; REST corepressor 1
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