Human/Mouse RIAM/APBB1IP Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects recombinant human RIAM/APBB1IP in direct ELISAs. Detects human and mouse RIAM/APBB1IP in Western blots
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human RIAM/APBB1IP
    Gly2-Ser89
    Accession # Q7Z5R6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse RIAM/APBB1IP by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIAM/APBB1IP at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
RIAM/APBB1IP in Wi-38 Human Cell Line. RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIAM/APBB1IP

APBB1IP (Amyloid beta A4 precursor protein-binding family B member 1 interacting protein; also PREL-1, Pro-rich protein 73, RARP1 and RIAM) is a member of the MRL family of proteins. Although its predicted MW is 73 kDa, it runs anomalously at 100-110 kDa in SDS-PAGE. It is expressed in megakaryocytes, T cells and fibroblasts. APBB1IP acts as a coordinator for Rap1 GTPase signaling. Rap1 both antagonizes Ras signaling, and is associated with beta 1 and beta 2 integrin-induced cell adhesion and cytoskeleton rearrangement. Within this context, APBB1IP operates as a cytosolic adaptor protein that induces integrin activation by recruiting talin and RAP1 to the cell membrane, and by mediating Profilin and VASP protein polymerizatiuon of actin. Human APBB1IP is 666 amino acids (aa) in length. It contains a poly-Pro sequence (aa 129-148), a Ras-association domain (aa 176-263), a PH domain (aa 310-419), and a large poly-Pro region (aa 503-640). There are multiple potential isoform variants. One contains a deletion of aa 152-177 coupled to a 27 aa substitution for aa 262-666, a second possesses a 21 aa substitution for aa 152‑666, and a third shows a deletion of aa 386-390 coupled to a five aa substitution for aa 417-666. Over aa 2-89, human APBB1IP shares 97% aa sequence identity with mouse APBB1IP.

  • Long Name:
    Amyloid Beta (A4) Precursor Protein-binding, Family B, Member 1 Interacting Protein
  • Entrez Gene IDs:
    54518 (Human); 54519 (Mouse); 307171 (Rat)
  • Alternate Names:
    amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein; amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; APBB1IP; INAG1; PREL1; PREL-1; proline rich EVH1 ligand 1; Proline-rich EVH1 ligand 1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Rap1-interacting adaptor molecule; RARP1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Cutting Edge: Loss of T Cell RIAM Precludes Conjugate Formation with APC and Prevents Immune-Mediated Diabetes
    Authors: F Lagarrigue, FB Gertler, MH Ginsberg, JM Cantor
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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