Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SEZ6L2/BSRP-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5

Specificity

Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse SEZ6L2/BSRP-A Antibody

SEZ6L2/BSRP-A antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

SEZ6L2/BSRP-A in Mouse Embryo.

SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse SEZ6L2/BSRP-A Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SEZ6L2/BSRP-A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEZ6L2/BSRP-A

SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.

Long Name

Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A

Alternate Names

BSRP-A, BSRPA, PSK-1

Entrez Gene IDs

26470 (Human); 233878 (Mouse); 308988 (Rat)

Gene Symbol

SEZ6L2

UniProt

Q4V9Z5 (Mouse)

Additional SEZ6L2/BSRP-A Products

Product Documents for Human/Mouse SEZ6L2/BSRP-A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SEZ6L2/BSRP-A Antibody

For research use only

Citations for Human/Mouse SEZ6L2/BSRP-A Antibody

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Protocols

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