Human/Mouse Sonic Hedgehog N‑Terminus Antibody
Human/Mouse Sonic Hedgehog N‑Terminus Antibody Summary
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Product Datasheets
Citations for Human/Mouse Sonic Hedgehog N‑Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Pancreatic tumor cell secreted CCN1/Cyr61 promotes endothelial cell migration and aberrant neovascularization.
Authors: Maity, Gargi, Mehta, Smita, Haque, Inamul, Dhar, Kakali, Sarkar, Sandipto, Banerjee, Sushanta, Banerjee, Snigdha
Sci Rep, 2014;4(0):4995.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury.
Authors: Hashimoto M, Ishii K, Nakamura Y, Watabe K, Kohsaka S, Akazawa C
J. Neurochem., 2008;107(4):918-27.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.
Authors: Banziger C, Soldini D, Schutt C, Zipperlen P, Hausmann G, Basler K
Cell, 2006;125(3):509-22.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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