Human/Mouse STAT3 Antibody

Detection of Human STAT3 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STAT3 at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STAT3 in Human PBMCs.

STAT3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Sheep Anti-Human STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17991) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human STAT3 by Western Blot

Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M  =  media, iso ctrl  =  isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.