Detects human and mouse TBX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross‑reactivity with recombinant human (rh) TBX2 is observed and less than 1% cross‑reactivity with rhTBX3, rhTBX6, rhTBX18, and rhTBX20 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human TBX5 Lys253-Lys327 Accession # Q99593
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse TBX5 by Western Blot. Western blot shows lysates of human heart and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TBX5 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human and Mouse TBX5 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for TBX5 at approximately 56 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TBX5 (T-box protein 5) is a 64 kDa member of the T-box family of transcriptional regulators. It is expressed in both embryo and adult, and is particularly important in the development of the heart and upper limb. TBX5 is a transcriptional activator and influences the expression of FGF‑10 and NPPA/ANF. Human TBX5 is 518 amino acids (aa) in length. It contains one T‑box DNA‑binding domain (aa 58‑238), a transactivation motif (Glu349GluAsp351), and an NLS (aa 339‑379). There are multiple splice forms. One 35 kDa isoform shows a RPLL substitution for aa 252‑518, generating a TBX5 antagonist. Other isoforms show an alternative start site at Met51, a 136 aa substitution for aa 445‑518, a 33 aa substitution for aa 360‑518, a 22 aa substitution for aa 328‑518, and a 41 aa substitution for aa 7‑49. Over aa 253‑327, human TBX5 shares 95% aa identity with mouse TBX5.
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