Human/Mouse TBX5 Antibody Summary
Accession # Q99593
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse TBX5 by Western Blot. Western blot shows lysates of human heart and mouse embryonic heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TBX5 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human and Mouse TBX5 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for TBX5 at approximately 56 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse TBX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TBX5 (T-box protein 5) is a 64 kDa member of the T-box family of transcriptional regulators. It is expressed in both embryo and adult, and is particularly important in the development of the heart and upper limb. TBX5 is a transcriptional activator and influences the expression of FGF‑10 and NPPA/ANF. Human TBX5 is 518 amino acids (aa) in length. It contains one T‑box DNA‑binding domain (aa 58‑238), a transactivation motif (Glu349GluAsp351), and an NLS (aa 339‑379). There are multiple splice forms. One 35 kDa isoform shows a RPLL substitution for aa 252‑518, generating a TBX5 antagonist. Other isoforms show an alternative start site at Met51, a 136 aa substitution for aa 445‑518, a 33 aa substitution for aa 360‑518, a 22 aa substitution for aa 328‑518, and a 41 aa substitution for aa 7‑49. Over aa 253‑327, human TBX5 shares 95% aa identity with mouse TBX5.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse TBX5 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse TBX5 Antibody and earn rewards!
Have you used Human/Mouse TBX5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image