Human/Mouse VAMP-1/VAMP-2 Antibody Summary
Accession # P23763
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1‑96) and a 22 aa transmembrane domain (aa 97‑118). There is one coiled-coil region between aa 33‑93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1‑96, human VAMP-1 is 98% aa identical to mouse VAMP-1.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse VAMP-1/VAMP-2 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse VAMP-1/VAMP-2 Antibody and earn rewards!
Have you used Human/Mouse VAMP-1/VAMP-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image