Human/Mouse VAMP-1/VAMP-2 Antibody

Catalog # Availability Size / Price Qty
MAB5958
MAB5958-SP
Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot.
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Human/Mouse VAMP-1/VAMP-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse VAMP-1/VAMP-2 in Western blots. This antibody does not react with recombinant VAMP family members 5, 7, and 8.
Source
Monoclonal Mouse IgG1 Clone # 540524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain (globus pallidus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse VAMP‑1/VAMP‑2 antibody by Western Blot. View Larger

Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-1/VAMP-2

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1‑96) and a 22 aa transmembrane domain (aa 97‑118). There is one coiled-coil region between aa 33‑93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1‑96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Long Name
Vesicle-Associated Membrane Protein 1/2
Entrez Gene IDs
6843 (Human); 22317 (Mouse); 25624 (Rat)
Alternate Names
SPAX1; SYB1; VAMP1; VAMP-1; VAMP-1/VAMP-2; vesicle associated membrane protein 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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