Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 341504
View all Wnt-8b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt‑8b

Specificity

Detects mouse Wnt-8b in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant mouse Wnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b,-10b, recombinant human Wnt-2, -7a, -7b, or -9a is observed. Immunohistochemical staining of the human neural cell line, NTera2, demonstrates that this antibody also recognizes human Wnt-8b.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse Wnt‑8b Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-8b

Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Alternate Names

Wnt8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Gene Symbol

WNT8B

Additional Wnt-8b Products

Product Documents for Human/Mouse Wnt‑8b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Wnt‑8b Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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Protocols

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FAQs

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