Human Nephrin Antibody

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Human Nephrin Antibody in Data
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Product Details
Citations (10)
Supplemental Products
Reviews (1)

Human Nephrin Antibody Summary

Species Reactivity
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Nephrin
Accession # O60500
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Nephrin antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Nephrin in Human Kidney. Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Nephrin antibody by Western Blot. View Larger

Detection of Human Nephrin by Western Blot. Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephrin

Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.

Entrez Gene IDs
4868 (Human); 54631 (Mouse); 64563 (Rat)
Alternate Names
CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor

Product Datasheets

Citations for Human Nephrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Transcription factor 21 expression in injured podocytes of glomerular diseases
    Authors: J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata
    Sci Rep, 2020;10(1):11516.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Evaluation of variability in human kidney organoids
    Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
    Nat. Methods, 2019;16(1):79-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Organoid single cell profiling identifies a transcriptional signature of glomerular disease
    Authors: JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,,
    JCI Insight, 2019;4(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation
    Authors: A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka
    Nat Commun, 2019;10(1):5462.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  5. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening
    Authors: LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little
    Nat Commun, 2018;9(1):5167.
    Species: Human
    Sample Types:
  6. Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics
    Authors: H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions
    Sci Rep, 2016;6(0):37172.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
    Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
    Nature, 2015;526(7574):564-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
    Authors: Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J
    Cell, 0;181(4):905-913.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  10. Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies.
    Authors: Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T
    Drug Metab Dispos, 0;46(11):1703-1711.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Human Nephrin Antibody
By Anonymous on 02/09/2021
Application: ICC/IF Sample Tested: Kidney tissue Species: Human