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Human Nephrin Antibody

Catalog #: AF4269
21 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4269
AF4269-SP
Detection of Human Nephrin by Western Blot.
3 Images
Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nephrin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nephrin
Gln23-Thr1029
Accession # O60500
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Human kidney
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Nephrin antibody by Western Blot. View Larger

Detection of Human Nephrin by Western Blot. Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Nephrin antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Nephrin in Human Kidney. Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Nephrin by Simple WesternTM. Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Nephrin at approximately 185 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephrin

Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.

Entrez Gene IDs
4868 (Human); 54631 (Mouse); 64563 (Rat)
Alternate Names
CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor

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Citations for Human Nephrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation
    Authors: G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink
    Cell Stem Cell, 2022;29(11):1580-1593.e7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days
    Authors: N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers
    Cells, 2022;11(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Transplanted organoids empower human preclinical assessment of drug candidate for the clinic
    Authors: AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel
    Science Advances, 2022;8(27):eabj5633.
    Species: Human
    Sample Types: Organoid
    Applications: Flow Cytometry
  4. TGF-beta1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction
    Authors: S Ueda, T Tominaga, A Ochi, A Sakurai, K Nishimura, E Shibata, S Wakino, M Tamaki, K Nagai
    Scientific Reports, 2021;11(1):21643.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
    Authors: J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann
    Cell Stem Cell, 2021;29(2):217-231.e8.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  6. Human kidney organoids reveal the role of glutathione in Fabry disease
    Authors: JW Kim, HW Kim, SA Nam, JY Lee, HJ Cho, TM Kim, YK Kim
    Experimental & Molecular Medicine, 2021;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host
    Authors: KS Lee, J Lee, P Lee, BC Jeon, MY Song, S Kwak, J Lee, JS Kim, DJ Kim, JH Kim, VL Tesh, MS Lee, SK Park
    Embo Molecular Medicine, 2021;0(0):e14678.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  8. Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis
    Authors: R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe
    Cell Stem Cell, 2021;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  9. Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis
    Authors: L Miesen, P Bándi, B Willemsen, F Mooren, T Strieder, E Boldrini, V Drenic, J Eymael, R Wetzels, J Lotz, N Weiss, E Steenberge, TH van Kuppev, M van Erp, J van der La, N Endlich, MJ Moeller, JFM Wetzels, J Jansen, B Smeets
    Disease Models & Mechanisms, 2021;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development
    Authors: N Mangold, J Pippin, D Unnersjoe-, S Koehler, S Shankland, S Brähler, B Schermer, T Benzing, PT Brinkkoett, H Hagmann
    Cells, 2021;10(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Transcription factor 21 expression in injured podocytes of glomerular diseases
    Authors: J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata
    Sci Rep, 2020;10(1):11516.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
    Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
    Cell Reports, 2020;33(11):108514.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  13. Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation
    Authors: A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka
    Nat Commun, 2019;10(1):5462.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  14. Organoid single cell profiling identifies a transcriptional signature of glomerular disease
    Authors: JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,,
    JCI Insight, 2019;4(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Evaluation of variability in human kidney organoids
    Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
    Nat. Methods, 2019;16(1):79-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics
    Authors: H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys
    Cell Stem Cell, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening
    Authors: LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little
    Nat Commun, 2018;9(1):5167.
    Species: Human
    Sample Types:
  18. ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions
    Sci Rep, 2016;6(0):37172.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
    Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
    Nature, 2015;526(7574):564-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies.
    Authors: Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T
    Drug Metab Dispos, 0;46(11):1703-1711.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
    Authors: Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J
    Cell, 0;181(4):905-913.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC

FAQs

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Human Nephrin Antibody
By Anonymous on 02/09/2021
Application: ICC/IF Sample Tested: Kidney tissue Species: Human
Immunocytochemistry/Immunofluorescence Human Nephrin Antibody AF4269