Human Nephrin Antibody
Human Nephrin Antibody Summary
Gln23-Thr1029
Accession # O60500
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Nephrin in Human Kidney. Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Nephrin by Western Blot. Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nephrin
Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.
Product Datasheets
Citations for Human Nephrin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Evaluation of variability in human kidney organoids
Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
Nat. Methods, 2019;16(1):79-87.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Organoid single cell profiling identifies a transcriptional signature of glomerular disease
Authors: JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,,
JCI Insight, 2019;4(1):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation
Authors: A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka
Nat Commun, 2019;10(1):5462.
Species: Mouse
Sample Types: Organoid
Applications: ICC -
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening
Authors: LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little
Nat Commun, 2018;9(1):5167.
Species: Human
Sample Types:
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Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics
Authors: H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys
Cell Stem Cell, 2018;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions
Sci Rep, 2016;6(0):37172.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
Nature, 2015;526(7574):564-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
Authors: Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J
Cell, 0;181(4):905-913.e7.
Species: Human
Sample Types: Whole Cells
Applications: IHC
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