Human Nephrin Antibody Summary
Gln23-Thr1029
Accession # O60500
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Human Nephrin by Western Blot. Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Nephrin in Human Kidney. Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Nephrin by Simple WesternTM. Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Nephrin at approximately 185 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nephrin
Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.
Product Datasheets
Citations for Human Nephrin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation
Authors: G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink
Cell Stem Cell, 2022;29(11):1580-1593.e7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days
Authors: N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers
Cells, 2022;11(11):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transplanted organoids empower human preclinical assessment of drug candidate for the clinic
Authors: AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel
Science Advances, 2022;8(27):eabj5633.
Species: Human
Sample Types: Organoid
Applications: Flow Cytometry -
TGF-beta1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction
Authors: S Ueda, T Tominaga, A Ochi, A Sakurai, K Nishimura, E Shibata, S Wakino, M Tamaki, K Nagai
Scientific Reports, 2021;11(1):21643.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids
Authors: J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann
Cell Stem Cell, 2021;29(2):217-231.e8.
Species: Human
Sample Types: Organoids
Applications: IHC -
Human kidney organoids reveal the role of glutathione in Fabry disease
Authors: JW Kim, HW Kim, SA Nam, JY Lee, HJ Cho, TM Kim, YK Kim
Experimental & Molecular Medicine, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host
Authors: KS Lee, J Lee, P Lee, BC Jeon, MY Song, S Kwak, J Lee, JS Kim, DJ Kim, JH Kim, VL Tesh, MS Lee, SK Park
Embo Molecular Medicine, 2021;0(0):e14678.
Species: Human
Sample Types: Organoids
Applications: IHC -
Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis
Authors: R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis
Authors: L Miesen, P Bándi, B Willemsen, F Mooren, T Strieder, E Boldrini, V Drenic, J Eymael, R Wetzels, J Lotz, N Weiss, E Steenberge, TH van Kuppev, M van Erp, J van der La, N Endlich, MJ Moeller, JFM Wetzels, J Jansen, B Smeets
Disease Models & Mechanisms, 2021;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development
Authors: N Mangold, J Pippin, D Unnersjoe-, S Koehler, S Shankland, S Brähler, B Schermer, T Benzing, PT Brinkkoett, H Hagmann
Cells, 2021;10(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transcription factor 21 expression in injured podocytes of glomerular diseases
Authors: J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata
Sci Rep, 2020;10(1):11516.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling
Authors: K Uchimura, H Wu, Y Yoshimura, BD Humphreys
Cell Reports, 2020;33(11):108514.
Species: Human
Sample Types: Organoid
Applications: IHC -
Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation
Authors: A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka
Nat Commun, 2019;10(1):5462.
Species: Mouse
Sample Types: Organoid
Applications: ICC -
Organoid single cell profiling identifies a transcriptional signature of glomerular disease
Authors: JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,,
JCI Insight, 2019;4(1):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Evaluation of variability in human kidney organoids
Authors: B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little
Nat. Methods, 2019;16(1):79-87.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics
Authors: H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys
Cell Stem Cell, 2018;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening
Authors: LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little
Nat Commun, 2018;9(1):5167.
Species: Human
Sample Types:
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ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions
Sci Rep, 2016;6(0):37172.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
Nature, 2015;526(7574):564-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies.
Authors: Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T
Drug Metab Dispos, 0;46(11):1703-1711.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
Authors: Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J
Cell, 0;181(4):905-913.e7.
Species: Human
Sample Types: Whole Cells
Applications: IHC
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