Human Neprilysin/CD10 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects Neprilysin/CD10 in ELISAs and Western blots. In sandwich ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) Neprilysin is observed and less than 0.3% cross-reactivity with recombinant human (rh) Neprilysin-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
    Tyr52-Trp750
    Accession # P08473
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC)
  • Flow Cytometry
    2.5 µg/106 cells
    CD19+ human peripheral blood lymphocytes
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Human Neprilysin/CD10 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Neprilysin/CD10 Antibody (Catalog # AF1182)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1182)
  • ELISA Standard
     
    Recombinant Human Neprilysin (CHO-expressed) Protein, CF (Catalog # 1182-ZNC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Neprilysin/CD10 in Human Kidney. Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human Neprilysin/CD10 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1182) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta  peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).

  • References:
    1. Malfroy, B. et al. (1978) Nature 276:523.
    2. Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
    3. LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
    4. Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
    5. Itwata, N. et al. (2001) Science 292:1550.
  • Entrez Gene IDs:
    4311 (Human); 17380 (Mouse); 24590 (Rat)
  • Alternate Names:
    Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase
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