Human Neudesin Antibody

Catalog # Availability Size / Price Qty
AF6714
AF6714-SP
Human Neudesin Antibody in Immunohistochemistry
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Human Neudesin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neudesin in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Neudesin
Gly32-Phe172
Accession # Q9UMX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Neudesin in Human Brain. View Larger

Neudesin in Human Brain. Neudesin was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human Neudesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6714) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neudesin

Neudesin (Neuron-derived neurotrophic secreted protein; also CIR2 and SPUF) is a secreted, 20-21 kDa member of the MAPR (membrane-associated progesterone receptor) subfamily, cytochrome b5 family of molecules. It is expressed by CNS neuronal progenitors and neurons, plus preadipocytes in white adipose tissue. Regarding activity, it promotes neuronal differentiation with limited proliferation, and serves as a neuron survival factor. By contrast, it inhibits both astrocyte and adipocyte differentiation. Mature human neudesin is 141 amino acids (aa) in length (aa 32-172). It contains one cytochrome b5-like heme-binding domain (aa 44-129) and an acetylated lysine residue at Lys136. The heme-binding domain does bind heme, and this accounts for 5-6 kDa of its circulating molecular weight. The presence of a heme moiety is necessary for activity. Mature human neudesin shares 97% aa identity with mature mouse neudesin.

Entrez Gene IDs
29937 (Human); 66208 (Mouse); 289380 (Rat)
Alternate Names
Cell immortalization-related protein 2; CIR2; CIR2cell growth-inhibiting protein 47; NENF; Neudesin; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; SCIRP10; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; SPUF protein; SPUF; SPUFneudesin

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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