Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all NeuroD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NeuroD1
Ser154-Asp356
Accession # Q13562

Specificity

Detects human NeuroD1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human NeuroD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human NeuroD1 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human NeuroD1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NeuroD1

NeuroD1, also known as beta-cell E-box transactivator 2 (Beta 2), is a basic helix-loop-helix transcription factor. It is expressed during development in various neurons of the central and peripheral nervous systems. NeuroD1 is also expressed in beta-cells in the pancreas where it functions both as a transcriptional activator and repressor.

Long Name

Neurogenic Differentiation Factor 1

Alternate Names

BETA2, BHF-1, NIDDM

Entrez Gene IDs

4760 (Human)

Gene Symbol

NEUROD1

UniProt

Q13562

Additional NeuroD1 Products

Product Documents for Human NeuroD1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NeuroD1 Biotinylated Antibody

For research use only

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Protocols

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FAQs

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