Human Neuroglycan C/CSPG5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Neuroglycan C/CSPG5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Neuroglycan C is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Neuroglycan C/CSPG5
    Val31-Gln420
    Accession # AAQ04776
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse brain (cerebellum)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Neuroglycan C/CSPG5 by Western Blot.

Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuroglycan C/CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5685) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Neuroglycan C/CSPG5 at approximately 120 and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuroglycan C/CSPG5
Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120‑150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1, 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2, 3). Human NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 34‑272), with CS attachment at Ser117, one EGF‑like domain (aa 371‑413), two potential sites for N‑linked glycosylation, and twelve potential sites for O‑linked glycosylation (4). Splicing variants produce three isoforms for human NGC. Isoform 1 is the long form. Isoform 2 has a deletion of aa 487‑513, while isoform 3 has an alternative start site at Met139 and the same deletion. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31‑420, human NGC shares 84% aa identity with mouse NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3‑kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin‑binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor‑like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).
  • References:
    1. Kinugasa, Y. et al. (2004) Biochem. Biophys. Res. Commun 321:1045.
    2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
    3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
    4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
    5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
    6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
    7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.
     
  • Entrez Gene IDs:
    10675 (Human); 29873 (Mouse); 50568 (Rat)
  • Alternate Names:
    CALEB; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; CSPG5; MGC44034; Neuroglycan C; NGC; NGCAcidic leucine-rich EGF-like domain-containing brain protein
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