Human Neuroligin 4X/NLGN4X Antibody

Catalog # Availability Size / Price Qty
Detection of Human Neuroligin 4X/NLGN4X by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human Neuroligin 4X/NLGN4X Antibody Summary

Species Reactivity
Detects human Neuroligin 4/NLGN4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NLGN3 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human Neuroligin 4/NLGN4
Accession # Q8N0W4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neuroligin 4X/NLGN4X antibody by Western Blot. View Larger

Detection of Human Neuroligin 4X/NLGN4X by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuroligin 4X/NLGN4X Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroligin 4X/NLGN4X at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry Neuroligin 4/NLGN4 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Neuroligin 4/NLGN4 in Human Brain. Neuroligin 4/NLGN4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and their processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 4X/NLGN4X

Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1‑3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X‑chromosome. It shares 69%‑73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta ‑neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium‑dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8‑10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).

  1. Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
  2. Chih, B. et al. (2005) Science 307:1324.
  3. Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
  4. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
  5. Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
  6. Fabrichny, I. P. et al. (2007) Neuron 56:979.
  7. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
  8. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  9. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  10. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
  11. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
Entrez Gene IDs
57502 (Human)
Alternate Names
ASPGX2; AUTSX2; HLNX; HNL4X; HNLX; KIAA1260AUTSX2; MGC22376; neuroligin 4, X-linked; Neuroligin 4X; NL4; NLGN; NLGN4; NLGN4neuroligin 4; NLGN4X; X-linked

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Citation for Human Neuroligin 4X/NLGN4X Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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