Human NM23-H2 Antibody Summary
Accession # P22392
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
NM23‑H2 in MCF‑7 Human Cell Line. NM23-H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human NM23-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6665) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human NM23‑H2 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NM23-H2 Polyclonal Antibody (Catalog # AF6665) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H2 at approximately 20 and 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NM23-H2 or NME-2 (non-metastatic cell 2; also Nucleoside diphosphate kinase B, nm23-H2/B, NDPK-B and PUF) is a 17-19 kDa class 1 member of the NME/NDPK family of molecules. It is widely expressed, found in both cytosol and nucleus, and participates in multiple activities. In conjunction with nm23-H1, NME-2 forms homo- and heterohexamers (three dimers or two trimers) that mediate the transfer of phosphate from ATP to nucleoside diphosphates. It also serves as a transcriptional regulator of genes such as c-myc and PDGF-A. Human NME-2 is 152 amino acids (aa) in length. It contains a kinase region (aa 5-134), two potential phosphorylation sites (Tyr52 and Thr94), a phosphohistidine residue at position 118, and multiple lysine acetylation sites. There is one potential NME-2 splice form that shows a six aa substitution for aa 77-152. The genes for NME-1 and NME-2 are adjacent, and an unusual splicing event generates a 33 kDa fusion protein (NM23-LV) that is composed of aa 1-114 of NME-1 plus a Thr insert that is N-terminally coupled to full-length NME-2. Full-length human NME-2 shares 88% and 98% aa identity with human NME-1 and mouse NME-2, respectively.
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