Human Nogo-A aa 566-748 Antibody

Catalog # Availability Size / Price Qty
Nogo‑A in Rat Cortical Stem Cells.
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Product Details
Citations (3)
Supplemental Products

Human Nogo-A aa 566-748 Antibody Summary

Species Reactivity
Detects human Nogo-A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant rat Nogo-A (aa 543-725) and recombinant human Nogo-B is observed..
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human Nogo-A
Accession # Q9NQC3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Nogo-A Fc Chimera, aa 566-748 (Catalog # 3515-NG)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nogo-A antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Nogo‑A in Rat Cortical Stem Cells. Nogo-A was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Sheep Anti-Human Nogo-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3515) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1-4). In human, Nogo exists in five isoforms (5-7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8-10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57-184 in human (aa 59-172 in rat) should block fibroblast spreading, while aa 566-748 in human (aa 544-725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13-15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005-1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16-18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566-748 segment.

  1. Oertle, T. et al. (2003) FASEB J. 17:1238.
  2. GrandPre, T. et al. (2000) Nature 403:439.
  3. Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
  4. Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
  5. Chen, M.S. et al. (2000) Nature 403:434.
  6. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
  7. GenBank Accession # Q9NQC3.
  8. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
  9. Fournier, A.E. et al. (2001) Nature 409:341.
  10. Wang, K.C. et al. (2002) Nature 420:74.
  11. Prinjha, R. et al. (2000) Nature 403:384.
  12. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  13. Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
  14. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
  15. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
  16. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
  17. Wang, X. et al. (2002) J. Neurosci. 22:5505.
  18. Acevedo, L. et al. (2004) Nat. Med. 10:382.
Long Name
Reticulon 4A
Entrez Gene IDs
57142 (Human); 68585 (Mouse); 83765 (Rat)
Alternate Names
ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

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Citations for Human Nogo-A aa 566-748 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration
    Authors: Y Sekine, JA Lindborg, SM Strittmatt
    Nat. Med., 2020;295(8):2175-2183.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
    Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
    PLoS ONE, 2017;12(12):e0189380.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Protein microarray analysis identifies cyclic nucleotide phosphodiesterase as an interactor of Nogo-A.
    Authors: Sumiyoshi K, Obayashi S, Tabunoki H
    Neuropathology, 2010;30(1):7-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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