Human Notch-2 Intracellular Domain Antibody (411801)

R&D Systems | Catalog # MAB3735

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 411801
View all Notch-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Notch‑2
Asp2063-Pro2413
Accession # Q04721.3

Specificity

Detects human Notch-2 intracellular domain (ICD) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Notch-1 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch‑4 ICD.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Notch-2 Intracellular Domain Antibody (411801)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Notch‑2 Intracellular Domain

Reviewed Applications

Read 3 reviews rated 3.7 using MAB3735 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins.  It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180 kDa disulfide-linked extracellular domain (ECD) (likely aa 25-1538) and a 110 kDa membrane bound truncated segment (likely aa 1539-2471).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa 1697-2471), a potential nuclear transcription factor.  In the nucleus, NICD regulates CSL pathways.  When NICD is phosphorylated, it is deactivated.  Over aa 2063-2413, human Notch-2 is 94% and 89% aa identical to dog and mouse Notch-2, respectively.   

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Gene Symbol

NOTCH2

UniProt

Q04721.3

Additional Notch-2 Products

Product Documents for Human Notch-2 Intracellular Domain Antibody (411801)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Notch-2 Intracellular Domain Antibody (411801)

For research use only

Customer Reviews for Human Notch-2 Intracellular Domain Antibody (411801) (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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  • Human Notch-2 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Notch-2 Intracellular Domain Antibody (411801) MAB3735
  • Human Notch-2 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Notch-2 Intracellular Domain Antibody (411801) MAB3735
  • Human Notch-2 Intracellular Domain Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018

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