Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant mouse Osteoprotegerin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
    Glu22-Leu401
    Accession # AAB53709
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Osteoprotegerin/TNFRSF11B (Catalog # 185-OS)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Osteoprotegerin/TNFRSF11B Antibody (Catalog # MAB8051)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody (Catalog # BAF805)
  • ELISA Standard
     
    Recombinant Human Osteoprotegerin/TNFRSF11B Protein (Catalog # 185-OS)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Osteoprotegerin/TNFRSF11B in MG-63 Human Cell Line.

Osteoprotegerin/TNFRSF11B was detected in immersion fixed MG-63 human osteosarcoma cell line using 10 µg/mL Goat Anti-Human Osteoprotegerin/
TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry
Osteoprotegerin/TNFRSF11B in Human Prostate.

Osteoprotegerin/TNFRSF11B was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell‑association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. Human OPG/OCIF cDNA encodes a 401 amino acid (aa) residues precursor protein with a 21 aa residue putative signal peptide that is removed to generate the mature soluble protein. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The 204 residues of the carboxy-terminal OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF kappa B ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1‑4. (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

  • Entrez Gene IDs:
    4982 (Human); 18383 (Mouse)
  • Alternate Names:
    OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects
    Authors: S Giovannini, G Tinelli, F Biscetti, G Straface, F Angelini, D Pitocco, L Mucci, R Landolfi, A Flex
    Cardiovasc Diabetol, 2017;16(1):99.
    Species: Human
    Sample Type: Serum
    Application: ELISA detection
  2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  3. The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
    Authors: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
    Cytokine, 2006;36(1):57-68.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005;32(4):713-20.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationELISA
Sample TestedPurified Standard
SpeciesHuman

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