Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF805
Osteoprotegerin/TNFRSF11B in MG-63 Human Cell Line.
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Product Details
Citations (5)
FAQs
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Reviews (1)

Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Osteoprotegerin/TNFRSF11B in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant mouse Osteoprotegerin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
Glu22-Leu401
Accession # AAB53709
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Osteoprotegerin/TNFRSF11B (Catalog # 185-OS)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Osteoprotegerin/TNFRSF11B Antibody (Catalog # MAB8051)

Standard: Recombinant Human Osteoprotegerin/TNFRSF11B Protein (Catalog # 185-OS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Osteoprotegerin/TNFRSF11B antibody in MG-63 Human Cell Line by Immunocytochemistry (ICC). View Larger

Osteoprotegerin/TNFRSF11B in MG-63 Human Cell Line. Osteoprotegerin/TNFRSF11B was detected in immersion fixed MG-63 human osteosarcoma cell line using 10 µg/mL Goat Anti-Human Osteoprotegerin/ TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Osteoprotegerin/TNFRSF11B antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

Osteoprotegerin/TNFRSF11B in Human Prostate. Osteoprotegerin/TNFRSF11B was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Osteoprotegerin/TNFRSF11B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF805) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG)/Osteoclastogenesis Inhibitory Factor (OCIF) is member of the tumor necrosis factor receptor superfamily that lacks any apparent cell‑association motifs and exists as a soluble secreted protein. In the new TNF superfamily nomenclature, OPG is referred to as TNFRSF11B. OPG was originally isolated by sequence homology as a TNF receptor family protein during a fetal rat intestine cDNA-sequencing project and subsequently shown to be involved in the regulation of bone density. OCIF was initially purified from the conditioned medium of human embryonic fibroblasts based on its ability to inhibit osteoclast development. Comparison of the amino-acid sequences of human OPG and OCIF proteins revealed their identity. Human OPG/OCIF cDNA encodes a 401 amino acid (aa) residues precursor protein with a 21 aa residue putative signal peptide that is removed to generate the mature soluble protein. The amino-terminal half of OPG contains four cysteine-rich repeats characteristic of TNF receptor family members. The 204 residues of the carboxy-terminal OPG/OCIF was found to contain two death domain homologous regions in tandem. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with the rat OPG. Natural OPG/OCIF has been found to exist predominantly as disulfide-linked dimers. Two TNF superfamily ligands, including the membrane proteins OPG ligand/TRANCE (tumor necrosis factor-related activation-induced cytokine)/ODF (osteoclast differentiation factor)/RANKL (receptor activator of NF kappa B ligand) and TRAIL (TNF-related apoptosis-inducing ligand)/APO-2 ligand, have been shown to be the cellular ligands for OPG/OCIF. Each of these ligands has been shown to interact with additional TNF receptor family members, including RANK (with TRANCE) and TRAIL receptors 1‑4. (with TRAIL). The roles of these receptor-ligands in osteoclastogenesis, apoptosis and in the immune system remains to be elucidated.

Entrez Gene IDs
4982 (Human); 18383 (Mouse)
Alternate Names
OCIF; OCIFMGC29565; OPG; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b

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Citations for Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Association between carotid plaque vulnerability and high mobility group box-1 serum levels in a diabetic population
    Authors: F Biscetti, G Tinelli, MM Rando, E Nardella, AL Cecchini, F Angelini, G Straface, M Filipponi, V Arena, D Pitocco, A Gasbarrini, M Massetti, A Flex
    Cardiovascular Diabetology, 2021;20(1):114.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  2. Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects
    Authors: S Giovannini, G Tinelli, F Biscetti, G Straface, F Angelini, D Pitocco, L Mucci, R Landolfi, A Flex
    Cardiovasc Diabetol, 2017;16(1):99.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  4. The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
    Authors: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
    Cytokine, 2006;36(1):57-68.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005;32(4):713-20.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Reviews for Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody

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Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody
By Anonymous on 06/29/2016
Application: ELISA Sample Tested: Purified Standard Species: Human