Human Osteoprotegerin/TNFRSF11B Antibody

Catalog # Availability Size / Price Qty
MAB8051-SP
MAB8051-500
MAB8051-100
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human Osteoprotegerin/TNFRSF11B Antibody Summary

Species Reactivity
Human
Specificity
Detects human Osteoprotegerin/TNFRSF11B in ELISAs. It has been reported (1), and R&D Systems testing has confirmed, that this antibody shows strong cross-reactivity with an unrelated protein, human Carbonic Anhydrase II. In Western blots, this antibody does not cross‑react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD30, rmCD40, rhDR3, rhDR6, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLT beta R, rhNGF R, rmOPG, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Source
Monoclonal Mouse IgG2A Clone # 69127
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
Glu22-Leu401
Accession # O00300
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Osteoprotegerin/TNFRSF11B Biotinylated Antibody (Catalog # BAF805)

Standard: Recombinant Human Osteoprotegerin/TNFRSF11B Protein (Catalog # 185-OS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin is a member of the TNF receptor superfamily expressed by many cell types. It exists in soluble monomeric and dimeric forms as a decoy receptor for its ligands, TRANCE and TRAIL. Osteoprotegerin functions as an antagonist to RANKL-induced osteoclastogenesis and bone resorption.

References
  1. Waterman, E.A. et al. (2007) Intl. J. Cancer 121:1958.
Entrez Gene IDs
4982 (Human); 18383 (Mouse)
Alternate Names
OCIF; OCIFMGC29565; OPG; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b

Product Datasheets

Citations for Human Osteoprotegerin/TNFRSF11B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Serum high mobility group box-1 and osteoprotegerin levels are associated with peripheral arterial disease and critical limb ischemia in type 2 diabetic subjects
    Authors: S Giovannini, G Tinelli, F Biscetti, G Straface, F Angelini, D Pitocco, L Mucci, R Landolfi, A Flex
    Cardiovasc Diabetol, 2017;16(1):99.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  3. Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis?
    Authors: Cross SS, Yang Z, Brown NJ, Balasubramanian SP, Evans CA, Woodward JK, Neville-Webbe HL, Lippitt JM, Reed MW, Coleman RE, Holen I
    Int. J. Cancer, 2006;118(8):1901-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
    Authors: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
    Cytokine, 2006;36(1):57-68.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K.
    Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT
    J. Rheumatol., 2005;32(4):713-20.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Osteoprotegerin/TNFRSF11B Antibody

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Human Osteoprotegerin/TNFRSF11B Antibody
By Anonymous on 07/19/2016
Application: ELISA Sample Tested: Serum and Plasma Species: Human