Ovastacin (ASTL, astacin-like metallo-endopeptidase and Oocyte astacin) is a 50 kDa member of the peptidase M12A family of proteins. Ovastacin is an oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization, it plays a role in the polyspermy inhibition. Human Ovastacin is a metalloendopeptidase that contains three zinc binding domains. Ovastacin cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. Over aa 24-431, human Ovastacin shares 68% aa identity with mouse Ovastacin.
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ovastacin
Ala24-Asp431
Accession # Q6HA08
Ala24-Asp431
Accession # Q6HA08
Specificity
Detects human Ovasticin in direct ELISAs and Western blots. In direct ELISAs, less then 1% cross-reactivity with recombinant human BMP-1 is observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Ovastacin Antibody
Detection of Human Ovastacin by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ovastacin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Ovastacin by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Ovastacin at approximately 69 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human Ovastacin Antibody
Application
Recommended Usage
Simple Western
1:100 dilution
Sample: K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line
Western Blot
1:1000 dilution
Sample: K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Ovastacin
Alternate Names
Astacin-Like Metalloendopeptidase, ASTL, Oocyte Astacin
Gene Symbol
ASTL
UniProt
Additional Ovastacin Products
Product Documents for Human Ovastacin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Ovastacin Antibody
For research use only
Related Research Areas
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