Human Ovastacin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Ovasticin in direct ELISAs and Western blots. In direct ELISAs, less then 1% cross-reactivity with recombinant human BMP-1 is observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Ovastacin
    Ala24-Asp431
    Accession # Q6HA08
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Simple Western
    1:100 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Ovastacin by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ovastacin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Ovastacin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR‑3  human ovarian carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Ovastacin at approximately 69  kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Ovastacin

Ovastacin (ASTL, astacin-like metallo-endopeptidase and Oocyte astacin) is a 50 kDa  member of the peptidase M12A family of proteins. Ovastacin is an oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization, it plays a role in the polyspermy inhibition. Human Ovastacin is a metalloendopeptidase that contains three zinc binding domains. Ovastacin cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. Over aa 24-431, human Ovastacin shares 68% aa identity with mouse Ovastacin.

  • Entrez Gene IDs:
    431705 (Human); 215095 (Mouse); 296129 (Rat)
  • Alternate Names:
    astacin-like metallo-endopeptidase (M12 family); Astacin-Like Metalloendopeptidase; ASTL; Oocyte Astacin; Ovastacin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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