Human p63/TP73L Biotinylated Antibody BAF1916: R&D Systems

Human p63/TP73L Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects p63/TP73L in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human p63/TP73L
    Met40-Cys339
    Accession # Q9H3D4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human p63/TP73L
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
p63/TP73L in Human Prostate. p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
p63/TP73L in SCC-25 Human Cell Line. p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

  • References:
    1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
    2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
    3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.
  • Long Name:
    Tumor Protein 63
  • Entrez Gene IDs:
    8626 (Human)
  • Alternate Names:
    amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like
Related Research Areas
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-Allophycocyanin

Flow F0050 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Avidin-Fluorescein

Flow F0030 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

MagCellect Streptavidin Ferrofluid

MAG999 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates

ELISA CP004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human p63/TP73L Biotinylated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information