Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all p63/TP73L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4

Specificity

Detects human p63/TP73L in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human p63/TP73L Antibody

p63/TP73L antibody in Human Breast by Immunohistochemistry (IHC-P).

p63/TP73L in Human Breast.

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

p63/TP73L antibody in SCC-25 Human Cell Line by Immunocytochemistry (ICC).

p63/TP73L in SCC-25 Human Cell Line.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse p63/TP73L by Immunocytochemistry/Immunofluorescence

Detection of Mouse p63/TP73L by Immunocytochemistry/Immunofluorescence

Analysis of p73 and p63 co-expression in human and murine skin.(A) Scatter plot of TP63 versus TP73 RNA-seq expression [units = transcripts per million (TPM)] by human tissue type (n = 37) from the Human Protein Atlas (172 total samples) [30]. Mean expression (TPM + 0.1) for each tissue is plotted on a log2 scale with a LOESS smooth local regression line (gray). Correlation between TP63 and TP73 was quantified using Spearman’s rank correlation coefficient (rs). (B) Representative micrographs of H&E and immunofluorescence (IF) staining on serial human (top) and mouse (bottom) skin sections; DAPI (blue), p63 alpha (green), and p73 (red). Regions of the skin in micrographs are labeled as: interfollicular epidermis (IFE), hair follicle (HF), outer root sheath (ORS), HF bulge (Bu), hair bulb (HB), sebaceous gland (SG), hair shaft (HS), and arrector pili muscle (APM). Scale bars represent 200 μm for human and 50 μm for murine tissue. See also S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human p63/TP73L by Western Blot

Detection of Human p63/TP73L by Western Blot

Analysis of delta Np73 in epidermal programming using an induced basal keratinocyte (iKC) model system.(A) Immunoblot of KLF4, p63 alpha, and p73 protein expression in neonatal human dermal fibroblast (HDFn) cells infected with lentivirus encoding delta Np73 isoforms ( delta Np73 alpha and delta Np73 beta ) or empty vector control in combination with KLF4 and delta Np63 alpha. Cells were grown for 3 days and protein was harvested for immunoblot analysis. (B) Bar graphs of RNA expression for the indicated keratinocyte genes in HDFn cells infected in (A). Cells were grown for 3 days and RNA was harvested for qRT-PCR analysis. Expression data are represented as the fold increase relative to control. The mean of three replicates is shown with error bars representing SEM. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. (C) Principal component analysis (PCA) plot of RNA-seq from HDFn cells infected with lentivirus encoding delta Np73 beta or empty vector control in combination with KLF4 and delta Np63 alpha. Cells were grown for 6 days and RNA was harvested for RNA-seq analysis. The percentage of variance contributed by each PC is listed in parentheses. (D and E) Tables listing the enriched Genome Ontology (GO) categories and pathways among the top 250 genes contributing to PC1 from (C). (F) Heatmap with the expression of a set of 44 genes that underlie the enrichment of GO categories from (D). Genes are annotated based on known roles in iKC-related processes (gray box) and the presence of a p63/p73 ChIP-seq peak within 50 kb of its TSS in multiple basal cell types (brown box). See also S5 and S6 Figs and S2–S9 Tables. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent Staining of Adult stem cell-derived Lung Organoids.

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue).

Applications for Human p63/TP73L Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed FaDu human hypopharyngeal carcinoma cell line and SCC-25 human tongue carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Western Blot

0.1 µg/mL
Sample: Recombinant Human p63/TP73L

Reviewed Applications

Read 4 reviews rated 4.3 using AF1916 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References

  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Long Name

Tumor Protein 63

Alternate Names

EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L

Entrez Gene IDs

8626 (Human)

Gene Symbol

TP63

UniProt

Q9H3D4

Additional p63/TP73L Products

Product Documents for Human p63/TP73L Antibody

Certificate of Analysis

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Product Specific Notices for Human p63/TP73L Antibody

For research use only

Citations for Human p63/TP73L Antibody

Customer Reviews for Human p63/TP73L Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Human p63/TP73L Antibody
    Name: Tony Reyes
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse lung paraffin embedded sections and mouse lung tissue
    Species: Mouse
    Verified Customer | Posted 09/23/2020
    p63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors.
    p63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors, using the Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) overnight at 4℃ in blocking buffer at a dilution of 1/400. Citrate buffer pH 6.0 was used for high temperature antigen retrieval for 15 minutes in a pressure cooker. Tissue was stained using a HRP-conjugated secondary antibody and DAB staining and counterstained with hematoxylin (blue). P63 positivity was seen in the basal cells found in the trachea of mice and in LSCC tumors but not in small cell lung cancer (SCLC) tumors from Rb1/p53/Myc and Rb1/p53/Rbl2 mice or in mucinous adenocarcinoma tumors found in SNL mice.
    Human p63/TP73L Antibody AF1916
  • Human p63/TP73L Antibody
    Name: Michele Torino
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/07/2017
  • Human p63/TP73L Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mammary gland tissue
    Species: Human
    Verified Customer | Posted 05/16/2017
  • Human p63/TP73L Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 12/15/2016
    p63 is prostate stem cell marker. i have isolated stem cells from BPH patients and stained with p63 marker. I found excellent results with DAB kit and also p63 antibody performed superb job.

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