Human p63/TP73L Antibody

(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human p63/TP73L in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human p63/TP73L
    Met40-Cys339
    Accession # Q9H3D4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human p63/TP73L
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
p63/TP73L in Human Breast. p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
p63/TP73L in SCC-25 Human Cell Line. p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

  • References:
    1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
    2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
    3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.
  • Long Name:
    Tumor Protein 63
  • Entrez Gene IDs:
    8626 (Human)
  • Alternate Names:
    amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like
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Average Rating: 4 (Based on 3 reviews)

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Read what people are saying who have used Human p63/TP73L Antibody.

We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 applications: Western Blot, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 WB Human Anonymous 12/07/2017
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Summary

ApplicationWestern Blot
Sample Tested293T human embryonic kidney cell line
SpeciesHuman
  Average
 WB Human Anonymous 05/16/2017
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Summary

ApplicationWestern Blot
Sample TestedMammary gland tissue
SpeciesHuman
  Excellent
 ICC/IF Human Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedProstate cancer
SpeciesHuman

Other Experimental Details

Other Experimental Detailsp63 is prostate stem cell marker. i have isolated stem cells from BPH patients and stained with p63 marker. I found excellent results with DAB kit and also p63 antibody performed superb job.

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