Human PDGF-BB Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF220
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human PDGF-BB Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF-BB in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat PDGF‑BB and porcine PDGF is observed, less than 2% cross-reactivity with recombinant human (rh) PDGF‑AB is observed, and less than 0.8% cross-reactivity with rhPDGF‑AA is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDGF‑BB (R&D Systems, Catalog # 220-BB)
Ser82-Thr190
Accession # Q6FHE7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF-BB (Catalog # 220-BB)

Human PDGF-BB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Recombinant Human PDGF R beta Fc Chimera Protein, CF (Catalog # 385-PR/CF)

Standard: Recombinant Human PDGF-BB Protein, CF (Catalog # 220-BB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-BB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Long Name
Platelet-derived Growth Factor BB
Entrez Gene IDs
5155 (Human); 24628 (Rat)
Alternate Names
PDGFBB; PDGF-BB

Product Datasheets

Citations for Human PDGF-BB Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
    Br. J. Cancer, 2012;107(10):1702-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: TaqMan Protein Assay
  2. Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release.
    Authors: Lacoste E, Martineau I, Gagnon G
    J. Periodontol., 2003;74(10):1498-507.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Reviews for Human PDGF-BB Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human PDGF-BB Biotinylated Antibody
By Anonymous on 07/19/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human PDGF-BB Biotinylated Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as a detection molecule in an sandwich ELISA measuring PDGF in human plasma samples.