Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha ‑granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.
Human PDGF-BB Biotinylated Antibody
R&D Systems | Catalog # BAF220
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Detection, ELISA Development, TaqMan Protein Assay
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human PDGF‑BB (R&D Systems, Catalog # 220-BB)
Ser82-Thr190
Accession # Q6FHE7
Ser82-Thr190
Accession # Q6FHE7
Specificity
Detects human PDGF-BB in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat PDGF‑BB and porcine PDGF is observed, less than 2% cross-reactivity with recombinant human (rh) PDGF‑AB is observed, and less than 0.8% cross-reactivity with rhPDGF‑AA is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PDGF-BB Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human PDGF-BB (Catalog # 220-BB)
Sample: Recombinant Human PDGF-BB (Catalog # 220-BB)
Human PDGF-BB Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using BAF220 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PDGF-BB
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
UniProt
Additional PDGF-BB Products
Product Documents for Human PDGF-BB Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PDGF-BB Biotinylated Antibody
For research use only
Related Research Areas
Citations for Human PDGF-BB Biotinylated Antibody
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Customer Reviews for Human PDGF-BB Biotinylated Antibody (3)
4.3 out of 5
3 Customer Ratings
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Application: Flow CytometrySample Tested: Purified PAbSpecies: Human PDGF-BB Biotinylated AffinityVerified Customer | Posted 10/13/2020Purified PA b, 50 UG f cyclic AMP micro domains in cardiac myocytesPurified PA b, 50 UG f cyclic AMP micro domains in cardiac myocytes
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 07/19/2019
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/16/2017This antibody was used as a detection molecule in an sandwich ELISA measuring PDGF in human plasma samples.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
