Human Phospho-Axl (Y779) Antibody

Catalog # Availability Size / Price Qty
AF2228
AF2228-SP
Phospho-Axl (Y779) in Human Stomach Cancer Tissue.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (3)

Human Phospho-Axl (Y779) Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse Axl when phosphorylated at Y779.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human Axl Y779 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Phospho-Axl (Y779) in Human Stomach Cancer Tissue. View Larger

Phospho-Axl (Y779) in Human Stomach Cancer Tissue. Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Phospho-Axl (Y779) by Western Blot. View Larger

Detection of Human Phospho-Axl (Y779) by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho‑Axl (Y779) Antigen Affinity‑purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho‑Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-Axl (Y779) in A172 Human Cell Line. View Larger

Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was detected using Rabbit Anti-Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D); Catalog # NL004) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C); Catalog # NL003). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL

Product Datasheets

Citations for Human Phospho-Axl (Y779) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC
    Authors: B Leonard, TM Brand, RA O'Keefe, E Lee, Y Zang, JD Kemmer, H Li, JR Grandis, NE Bhola
    Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury
    Authors: YL Chen, YT Tsai, TT Chao, YN Wu, MC Chen, YH Lin, CH Liao, SP Chou, HS Chiang
    Oncotarget, 2018;9(5):6402-6415.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  3. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017;117(12):1787-1797.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL
    Authors: K Iida, R Sakai, S Yokoyama, N Kobayashi, S Togo, HY Yoshikawa, A Rawangkan, K Namiki, M Suganuma
    Sci Rep, 2017;7(1):17770.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  5. Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells
    Authors: H Shin, D Kim, DM Helfman
    Oncotarget, 2017;8(56):95192-95205.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  6. Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
    Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
    Sci Rep, 2017;7(1):10613.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia.
    Authors: Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M
    Leukemia, 2015;29(12):2382-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  8. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  9. The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy.
    Authors: Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE
    Blood, 2011;117(6):1928-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  10. Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice.
    Authors: Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A
    J. Hepatol., 2009;51(1):55-66.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-Axl (Y779) Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human Phospho-Axl (Y779) Antibody
By Anonymous on 06/23/2017
Application: WB Sample Tested: Brain tissue Species: Mouse

Human Phospho-Axl (Y779) Antibody
By Anonymous on 10/06/2016
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line,PC-3 human prostate cancer cell line,DU145 human prostate carcinoma cell line,LNCaP human prostate cancer cell line,S521,S156,U-87 MG human glioblastoma/astrocytoma cell line,U-118-MG human glioblastoma/astrocytoma cell line,RKO,KHT,SCC-7 Species: Mouse,Human

Human Phospho-Axl (Y779) Antibody
By Anonymous on 04/25/2016
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,mcf10aDCIS.com,MDA-MB-231 human breast cancer cell line Species: Human