Human Phospho-Axl (Y779) Antibody
Human Phospho-Axl (Y779) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Phospho-Axl (Y779) in Human Stomach Cancer Tissue. Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Phospho-Axl (Y779) by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was detected using Rabbit Anti-Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D); Catalog # NL004) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C); Catalog # NL003). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axl
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.
- Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Holland, S. et al. (2005) Canc. Res. 65:9294.
Product Datasheets
Citations for Human Phospho-Axl (Y779) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
Authors: Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu
Cell Death & Disease, 2022;13(8):749.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The first laminin G-like domain of protein S is essential for binding and activation of Tyro3 receptor and intracellular signalling
Authors: N Al Kafri, J Ahnström, A Teraz-Oros, L Chaput, N Singh, BO Villoutrei, S Hafizi
Biochemistry and Biophysics Reports, 2022;30(0):101263.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis
Authors: M Jerkic, ML Litvack, S Gagnon, G Otulakowsk, H Zhang, O Rotstein, BP Kavanagh, M Post, JG Laffey
International Journal of Molecular Sciences, 2021;22(6):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
The miR?625?3p/AXL axis induces non?T790M acquired resistance to EGFR?TKI via activation of the TGF?&beta/Smad pathway and EMT in EGFR?mutant non?small cell lung cancer
Authors: W Du, L Sun, T Liu, J Zhu, Y Zeng, Y Zhang, X Wang, Z Liu, JA Huang
Oncol. Rep., 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tumour-Secreted Protein S (ProS1) Activates a Tyro3-Erk Signalling Axis and Protects Cancer Cells from Apoptosis
Authors: N Al Kafri, S Hafizi
Cancers (Basel), 2019;11(12):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Osimertinib and pterostilbene in EGFR-mutation-positive non-small cell lung cancer (NSCLC)
Authors: JWP Bracht, N Karachalio, J Berenguer, C Pedraz-Val, M Filipska, C Codony-Ser, J Codony-Ser, R Rosell
Int. J. Biol. Sci., 2019;15(12):2607-2614.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis
Authors: JP Hoj, B Mayro, AM Pendergast
Cell Rep, 2019;29(11):3421-3434.e8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Collaboration Between RSK-EphA2 and Gas6-Axl RTK Signaling in Arginine Starvation Response That Confers Resistance to EGFR Inhibitors
Authors: MT Kuo, Y Long, WB Tsai, YY Li, HHW Chen, LG Feun, N Savaraj
Transl Oncol, 2019;13(2):355-364.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
Authors: W Li, A Dick, F Lu, H Zhang, H Sun
Sci Rep, 2019;9(1):1988.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells
Authors: LC Cheng, YL Chen, AN Cheng, AY Lee, CY Cho, JS Huang, SE Chuang
J. Biomed. Sci., 2018;25(1):80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
AXL Mediates Esophageal Adenocarcinoma Cell Invasion through Regulation of Extracellular Acidification and Lysosome Trafficking
Authors: S Maacha, J Hong, A von Lersne, A Zijlstra, A Belkhiri
Neoplasia, 2018;20(10):1008-1022.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury
Authors: YL Chen, YT Tsai, TT Chao, YN Wu, MC Chen, YH Lin, CH Liao, SP Chou, HS Chiang
Oncotarget, 2018;9(5):6402-6415.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC
Authors: B Leonard, TM Brand, RA O'Keefe, E Lee, Y Zang, JD Kemmer, H Li, JR Grandis, NE Bhola
Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
Br. J. Cancer, 2017;117(12):1787-1797.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
Sci Rep, 2017;7(1):10613.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells
Authors: H Shin, D Kim, DM Helfman
Oncotarget, 2017;8(56):95192-95205.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL
Authors: K Iida, R Sakai, S Yokoyama, N Kobayashi, S Togo, HY Yoshikawa, A Rawangkan, K Namiki, M Suganuma
Sci Rep, 2017;7(1):17770.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia.
Authors: Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M
Leukemia, 2015;29(12):2382-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
J. Exp. Med., 2012;209(11):2033-47.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy.
Authors: Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE
Blood, 2011;117(6):1928-37.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice.
Authors: Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A
J. Hepatol., 2009;51(1):55-66.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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