Human Axl Antibody

Catalog # Availability Size / Price Qty
AF154
AF154-SP
Detection of Human Axl by Western Blot.
5 Images
Product Details
Citations (34)
FAQs
Supplemental Products
Reviews (3)

Human Axl Antibody Summary

Species Reactivity
Human
Specificity
Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Axl
Glu33-Pro440
Accession # AAA61243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-10 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable inAxl knockout A431 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Axl by Western Blot. View Larger

Detection of Human Axl by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Flow Cytometry Detection of Axl in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Axl in Human Stomach. View Larger

Axl in Human Stomach. Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Knockout Validated Western Blot Shows Human Axl Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Axl Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431cell line, but is not detectable in knockout A431 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL

Product Datasheets

Citations for Human Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts
    Authors: KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh
    Sci Rep, 2019;9(1):4762.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma
    Authors: L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham
    Mol. Cancer Ther., 2019;18(2):278-288.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018;515(0):176-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer
    Authors: Q Guo, T Grimmig, G Gonzalez, A Giobbie-Hu, G Berg, N Carr, BJ Wilson, P Banerjee, J Ma, JS Gold, B Nandi, Q Huang, AM Waaga-Gass, CG Lian, GF Murphy, MH Frank, M Gasser, NY Frank
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
    Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
    Sci Rep, 2017;7(1):10613.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. TAM Receptors Are Not Required for Zika Virus Infection in Mice
    Authors: AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki
    Cell Rep, 2017;19(3):558-568.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
    Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
    Sci Rep, 2017;7(0):41389.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196
    Authors: NB Leighl, MS Tsao, G Liu, D Tu, C Ho, FA Shepherd, N Murray, JR Goffin, G Nicholas, S Sakashita, Z Chen, L Kim, J Powers, L Seymour, G Goss, PA Bradbury
    Oncotarget, 2017;8(41):69651-69662.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
    Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017;117(12):1787-1797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses
    Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016;291(20):10586-601.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies
    Authors: Mihalis S Kariolis
    J. Clin. Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Zika virus cell tropism in the developing human brain and inhibition by azithromycin
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14408-14413.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex
    Authors: Zhi-Min Yuan
    J Mol Cell Biol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression
    Authors: K Sato, K Suda, S Shimizu, K Sakai, H Mizuuchi, K Tomizawa, T Takemoto, K Nishio, T Mitsudomi
    PLoS ONE, 2016;11(4):e0154186.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression
    Int J Mol Sci, 2016;18(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors
    EMBO Mol Med, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer
    Authors: LM Divine, MR Nguyen, E Meller, RA Desai, B Arif, EB Rankin, KH Bligard, C Meyerson, IS Hagemann, M Massad, PH Thaker, AR Hagemann, CK McCourt, MA Powell, DG Mutch, KC Fuh
    Oncotarget, 2016;7(47):77291-77305.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels.
    Authors: Fritz H, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlback B
    PLoS ONE, 2015;10(8):e0135991.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation.
    Authors: Rho, Jin Kyun, Choi, Yun Jung, Kim, Seon Ye, Kim, Tae Won, Choi, Eun Kyun, Yoon, Seon-Joo, Park, Bu Man, Park, Eunhye, Bae, Jong Hwa, Choi, Chang-Mi, Lee, Jae Cheo
    Cancer Res, 2014;74(1):253-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer.
    Authors: Ji, Wonjun, Choi, Chang-Mi, Rho, Jin Kyun, Jang, Se Jin, Park, Young So, Chun, Sung-Min, Kim, Woo Sung, Lee, Jung-Shi, Kim, Sang-We, Lee, Dae Ho, Lee, Jae Cheo
    BMC Cancer, 2013;13(0):606.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus.
    Authors: Migdall-Wilson J, Bates C, Schlegel J, Brandao L, Linger RM, Deryckere D, Graham DK
    PLoS ONE, 2012;7(2):e31635.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
    Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
    J. Virol., 2012;86(4):2067-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.
    Authors: Zhang Z, Lee JC
    Nat. Genet., 2012;44(8):852-860.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  29. Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.
    Authors: Brown JE, Krodel M, Pazos M
    PLoS ONE, 2012;7(5):e36800.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  30. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
    Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
    Thromb. Haemost., 2011;107(1):140-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
    Authors: Butler GS, Dean RA, Tam EM, Overall CM
    Mol. Cell. Biol., 2008;28(15):4896-914.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
    Authors: Uehara H, Shacter E
    J. Immunol., 2008;180(4):2522-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells.
    Authors: Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE
    Circ. Res., 2007;100(4):502-9.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  34. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Axl Antibody

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Human Axl Antibody
By Anonymous on 01/04/2019
Application: WB Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

Human Axl Antibody
By Anonymous on 06/22/2017
Application: ICC/IF Sample Tested: Neural progenitor cells Species: Human

Human Axl Antibody
By Anonymous on 01/20/2017
Application: Flow Sample Tested: AML cells Species: Human