Human Axl Antibody

Name: Human Axl Antibody
Brand: R&D Systems
Rating: 4 (2 reviews)

(30 citations)

(2 Reviews)

Datasheet / CoA / SDS

Western Blot

View Details

Flow Cytometry

View Details

Immunohistochemistry

View Details

Western Blot Shows Human Axl Specificity by Using Knockout Cell Line.

Knockout Validated

View Details

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Knockout Validated

View Details

Product Details

Citations (30)

FAQs

Supplemental Products

Reviews

Species Reactivity

Human
Specificity

Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human Axl
Glu33-Pro440
Accession # AAA61243
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

See below
Flow Cytometry

0.25 µg/10⁶ cells

See below
Immunohistochemistry

5-15 µg/mL

See below
Blockade of Receptor-ligand Interaction

In a functional ELISA, 3-10 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Knockout Validated

Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable inAxl knockout A431 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Human Axl by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Flow Cytometry click image to view larger	Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry click image to view larger	Axl in Human Stomach. Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Knockout Validated click image to view larger	Western Blot Shows Human Axl Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431cell line, but is not detectable in knockout A431 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated click image to view larger	Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References:
1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name:

Axl Receptor Tyrosine Kinase

Entrez Gene IDs:

558 (Human); 26362 (Mouse)

Alternate Names:

Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
Filter your results:

Species	Applications	Sample Type
Human Sertoli cells support high levels of Zika virus replication and persistence Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman Sci Rep, 2018;8(1):5477. Species: Human Sample Type: Whole Cells Application: Neut Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay Br. J. Cancer, 2017;117(12):1787-1797. Species: Human Sample Type: Whole Cells Application: Flow A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196 Authors: NB Leighl, MS Tsao, G Liu, D Tu, C Ho, FA Shepherd, N Murray, JR Goffin, G Nicholas, S Sakashita, Z Chen, L Kim, J Powers, L Seymour, G Goss, PA Bradbury Oncotarget, 2017;8(41):69651-69662. Species: Human Sample Type: Whole Tissue Application: IHC Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura Sci Rep, 2017;7(1):10613. Species: Human Sample Type: Cell Lysates Application: WB Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams J. Immunol., 2017;0(0):. Species: Human Sample Type: Tissue Homogenates Application: WB TAM Receptors Are Not Required for Zika Virus Infection in Mice Authors: AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki Cell Rep, 2017;19(3):558-568. Species: Human Sample Type: Cell Culture Supernates Application: WB AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029. Species: Human Sample Type: Whole Cells Application: Neut Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse Sci Rep, 2017;7(0):41389. Species: Human Sample Type: Whole Cells Application: ICC AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex Authors: Zhi-Min Yuan J Mol Cell Biol, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Zika virus cell tropism in the developing human brain and inhibition by azithromycin Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14408-14413. Species: Human Sample Type: Whole Cells Application: Neut Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies Authors: Mihalis S Kariolis J. Clin. Invest, 2016;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck J. Biol. Chem., 2016;291(20):10586-601. Species: Human Sample Type: Cell Lysates Application: WB AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer Authors: LM Divine, MR Nguyen, E Meller, RA Desai, B Arif, EB Rankin, KH Bligard, C Meyerson, IS Hagemann, M Massad, PH Thaker, AR Hagemann, CK McCourt, MA Powell, DG Mutch, KC Fuh Oncotarget, 2016;7(47):77291-77305. Species: Human Sample Type: Whole Tissue Application: IHC Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression Int J Mol Sci, 2016;18(1):. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors EMBO Mol Med, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression Authors: K Sato, K Suda, S Shimizu, K Sakai, H Mizuuchi, K Tomizawa, T Takemoto, K Nishio, T Mitsudomi PLoS ONE, 2016;11(4):e0154186. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers J Clin Invest, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels. Authors: Fritz H, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlback B PLoS ONE, 2015;10(8):e0135991. Species: Human Sample Type: Whole Cells Application: Flow MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation. Authors: Rho, Jin Kyun, Choi, Yun Jung, Kim, Seon Ye, Kim, Tae Won, Choi, Eun Kyun, Yoon, Seon-Joo, Park, Bu Man, Park, Eunhye, Bae, Jong Hwa, Choi, Chang-Mi, Lee, Jae Cheo Cancer Res, 2014;74(1):253-62. Species: Human Sample Type: Cell Lysates Application: WB Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer. Authors: Ji, Wonjun, Choi, Chang-Mi, Rho, Jin Kyun, Jang, Se Jin, Park, Young So, Chun, Sung-Min, Kim, Woo Sung, Lee, Jung-Shi, Kim, Sang-We, Lee, Dae Ho, Lee, Jae Cheo BMC Cancer, 2013;13(0):606. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry. Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y J. Virol., 2012;86(4):2067-78. Species: Human Sample Type: Whole Cells Application: Flow Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus. Authors: Migdall-Wilson J, Bates C, Schlegel J, Brandao L, Linger RM, Deryckere D, Graham DK PLoS ONE, 2012;7(2):e31635. Species: Human Sample Type: Cell Lysates Application: WB Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells. Authors: Brown JE, Krodel M, Pazos M PLoS ONE, 2012;7(5):e36800. Species: Human Sample Type: Cell Lysates Application: IP Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer. Authors: Zhang Z, Lee JC Nat. Genet., 2012;44(8):852-860. Species: Human Sample Type: Cell Lysates Application: IP Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. Species: Human Sample Type: Whole Cells Application: Neut Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Thromb. Haemost., 2011;107(1):140-9. Species: Human Sample Type: Whole Cells Application: ICC Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Authors: Butler GS, Dean RA, Tam EM, Overall CM Mol. Cell. Biol., 2008;28(15):4896-914. Species: Human Sample Type: Cell Lysates Application: WB Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells. Authors: Uehara H, Shacter E J. Immunol., 2008;180(4):2522-30. Species: Human Sample Type: Cell Lysates Application: WB Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Authors: Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE Circ. Res., 2007;100(4):502-9. Species: Bovine Sample Type: Cell Lysates Application: IP Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA J. Thromb. Haemost., 2005;3(4):733-41. Species: Human Sample Type: Whole Cells Application: Flow Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Description Application Cat# Citations Images

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

Expedite Your Research

Award-Winning Interactive Pathways

Posters, Brochures, Whitepapers & Reference Guides

Experts in Reagent Quality

Our People are Our Greatest Asset

You are here

Human Axl Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Basic

VisUCyte Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Summary

Summary

Order Details

Feedback

Reconstitution Calculator