Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 869201
View all EGFR Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human EGFR Y1197 site

Specificity

Detects human EGFR when phosphorylated at Y1197 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Phospho-EGFR (Y1197) Antibody

Detection of Human Phospho-EGFR (Y1197) antibody by Western Blot.

Detection of Human Phospho-EGFR (Y1197) by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8058) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1197) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-EGFR (Y1197) Antibody

Application
Recommended Usage

Western Blot

0.05 µg/mL
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human EGF (Catalog # 236-EG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGFR

EGF receptor, also known as ErbB1, is an approximately 160 kDa transmembrane receptor tyrosine kinase that binds multiple EGF family proteins. Ligand binding induces EGFR homodimerization or heterodimerization with ErbB2, 3,or 4 as well as activation of its kinase domain and phosphorylation within the cytoplasmic domain. Phosphorylation of Tyr869 by Src is important for full activation of the receptor. Phosphorylation of Tyr1197 by MAP kinases contributes to EGFR interaction with PIK3C2B. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Three additional alternative splice forms lack the transmembrane and cytoplasmic domains. Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human Phospho-EGFR (Y1197) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-EGFR (Y1197) Antibody

For research use only

Citations for Human Phospho-EGFR (Y1197) Antibody

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