Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1013F
View all MSK1/MSK2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human MSK1(S376) site.
Accession # O75582

Specificity

Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-MSK1(S376)/MSK2(S360) Antibody

Phospho-MSK1(S376)/MSK2(S360) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Phospho-MSK1(S376)/MSK2(S360) in HeLa Human Cell Line.

MSK1/2 phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated (top panel), or unstimulated (bottom panel), with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human MSK1/2 Monoclonal Antibody (Catalog # MAB25181) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Phospho-MSK1(S376)/MSK2(S360) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Phospho-MSK1(S376)/MSK2(S360) in HeLa Human Cell Line.

MSK1/2 phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with PMA using Rabbit Anti-Human MSK1/MSK2 Monoclonal Antibody (Catalog # MAB25181) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-MSK1(S376)/MSK2(S360) Antibody

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) or PMA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSK1/MSK2

Mitogen- and Stress-activated protein Kinases 1 and 2 (MSK1/2) have been shown to play key roles in the transcriptional regulation of immediate early genes such as c-fos. MSK1, also known as Ribosomal Protein S6 Kinase 5 (RPS6KA5), and MSK2, also known as RSKB and RPS6KA4, belong to the AGC family of kinases. Both proteins have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB. The phosphorylation of MSK1 at Ser376 or the equivalent Ser360 in MSK2 is required for kinase activity. These sites are located in the AGC kinase domain and are autophosphorylated. Their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. The sequence surrounding MSK1(S376) and MSK2(S360) is 100% identical. 

Long Name

Mitogen- and Stress-activated Protein Kinase 1 and 2

Alternate Names

1110069D02Rik, 3110005L17Rik, 6330404E13Rik

UniProt

O75582

Additional MSK1/MSK2 Products

Product Documents for Human Phospho-MSK1(S376)/MSK2(S360) Antibody

Certificate of Analysis

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Product Specific Notices for Human Phospho-MSK1(S376)/MSK2(S360) Antibody

For research use only

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