Human Phospho-MSK1(S376)/MSK2(S360) Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human MSK1 and MSK2 when phosphorylated at S376 and S360, respectively.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1013F
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human MSK1(S376) site.
    Accession # O75582
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Phospho-MSK1(S376)/MSK2(S360) in HeLa Human Cell Line. MSK1/2phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated (top panel), or unstimulated (bottom panel), with 200 ng/mL Recombinant Human EGF (Catalog # 236-EG) using Rabbit Anti-Human MSK1/2 Monoclonal Antibody (Catalog # MAB25181) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Phospho-MSK1(S376)/MSK2(S360) in HeLa Human Cell Line. MSK1/2phosphorylated at S376/S360 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with PMA using Rabbit Anti-Human MSK1/MSK2 Monoclonal Antibody (Catalog # MAB25181) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK1/MSK2
Mitogen- and Stress-activated protein Kinases 1 and 2 (MSK1/2) have been shown to play key roles in the transcriptional regulation of immediate early genes such as c-fos. MSK1, also known as Ribosomal Protein S6 Kinase 5 (RPS6KA5), and MSK2, also known as RSKB and RPS6KA4, belong to the AGC family of kinases. Both proteins have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB. The phosphorylation of MSK1 at Ser376 or the equivalent Ser360 in MSK2 is required for kinase activity. These sites are located in the AGC kinase domain and are autophosphorylated. Their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. The sequence surrounding MSK1(S376) and MSK2(S360) is 100% identical. 
  • Long Name:
    Mitogen- and Stress-activated Protein Kinase 1 and 2
  • Alternate Names:
    MSK1/MSK2
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