Human Phospho-STAT2 (Y689) Antibody

Catalog # Availability Size / Price Qty
AF2890
AF2890-SP
Detection of Phospho-STAT2 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry.
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Human Phospho-STAT2 (Y689) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT2 when phosphorylated at Y689 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human STAT2 Y689 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT2 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-STAT2 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1, dark orange filled histogram) for 20 minutes, then stained with Rabbit Anti-Human Phospho‑STAT2 (Y689) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2890) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Western Blot Detection of Human Phospho-STAT2 (Y689) by Western Blot. View Larger

Detection of Human Phospho-STAT2 (Y689) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human
IFN-alpha 2a (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog AF2890), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT2 (Y689) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human Phospho-STAT2 (Y689) by Simple WesternTM. View Larger

Detection of Human Phospho-STAT2 (Y689) by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y689) at approximately 111 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT2

STAT2 (signal transducer and activator of transcription #2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an
N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name
Signal Transducer and Activator of Transcription 2
Entrez Gene IDs
6773 (Human); 20847 (Mouse)
Alternate Names
interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2

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