Human Phospho-STAT5a/b (Y694/Y699) Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB4190S Datasheet / COA / SDS

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FAB4190S-100UG

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Human Phospho-STAT5a/b (Y694/Y699) Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT5a/b when phosphorylated at Y694/Y699 in Western blots.

Source

Monoclonal Rat IgG2a Clone # 941212

Purification

Protein A or G purified

Immunogen

Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694).
Accession # P51692

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: STAT5a

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN₃GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name

Signal Transducer and Activator of Transcription 5

Entrez Gene IDs

6776 (Human); 20850 (Mouse)

Alternate Names

MGF; signal transducer and activator of transcription 5A; STAT5; STAT5a