Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 941212
View all STAT5a/b Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694).
Accession # P51692

Specificity

Detects human STAT5a/b when phosphorylated at Y694/Y699 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Detection of Human Phospho-STAT5a/b (Y694/Y699) antibody by Western Blot.

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT5a/b (Y694/Y699) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-STAT5a/b (Y694/Y699) antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Phospho-STAT5a/b (Y694/Y699) in HeLa Human Cell Line.

STAT5a/b phosphorylated at Y694/Y699 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB4190) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Application
Recommended Usage

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1)

Reviewed Applications

Read 1 review rated 5 using MAB4190 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT5a/b

Signal Transduction and Activator of Transcription 5B (STAT5b) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN alpha ). Upon cytokine activation, STAT5b is phosphorylated and translocates to the nucleus where it binds to the DNA consensus site TTCN3GAA and activates transcription of specific genes. STAT5b shares 93% amino acid identity with STAT5a. STAT5b is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

MGF, STAT5, STAT5A signal transducer and activator of transcription 5A

UniProt

P51692

Additional STAT5a/b Products

Product Documents for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-STAT5a/b (Y694/Y699) Antibody

For research use only

Citations for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Customer Reviews for Human Phospho-STAT5a/b (Y694/Y699) Antibody (1)

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  • Human Phospho-STAT5a/b (Y694/Y699) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/04/2022
    Human Phospho-STAT5a/b (Y694/Y699) Antibody MAB4190

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Protocols

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