Human Phospho-TOR (S2448) Antibody

Catalog #: MAB1665
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1665
MAB1665-SP

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Detection of Human Phospho-TOR (S2448) by Western Blot.

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Citations (4)

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Human Phospho-TOR (S2448) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-TOR (S2448) in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 834115

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human TOR S2448 site
Accession # P42345

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-TOR (S2448) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TOR

The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival.

Long Name

Mammalian Target of Rapamycin

Entrez Gene IDs

2475 (Human); 287871 (Rat)

Alternate Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human Phospho-TOR (S2448) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis
Authors: M Schilling, AB Prusty, B Boysen, FS Oppermann, YL Riedel, A Husedzinov, H Rasouli, A König, P Ramanathan, J Reymann, H Erfle, H Daub, U Fischer, OJ Gruss
Cell Reports, 2021-06-22;35(12):109277.
Species: Human
Sample Types: Whole Cells
Applications: IHC, Western Blot
Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey
Authors: MT Lane, TJ Herda, AC Fry, MA Cooper, MJ Andre, PM Gallagher
Biol Sport, 2017-01-20;34(2):197-203.
Species: Human
Sample Types: Tissue Homogenates
Applications: Infared Microscopy, Western Blot
Theaflavin-3, 3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and Notch-1 pathways, not via MAPK pathways.
Authors: Gao Y, Rankin G, Tu Y, Chen Y
Int J Oncol, 2015-11-20;48(1):281-92.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma
Authors: Jacob Thomsen, Rikke Hjortebjerg, Ulrick Espelund, Gitte Ørtoft, Poul Vestergaard, Nils E. Magnusson et al.
Oncotarget
Species: Human
Sample Types: Ascites Fluid
Applications: Western Blot