Human Phospho-TOR (S2448) Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Phospho-TOR (S2448) in ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 834115
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human TOR S2448 site
    Accession # P42345
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-TOR (S2448) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOR
The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival.
  • Long Name:
    Mammalian Target of Rapamycin
  • Entrez Gene IDs:
    2475 (Human); 287871 (Rat)
  • Alternate Names:
    EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Theaflavin-3, 3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and Notch-1 pathways, not via MAPK pathways.
    Authors: Gao Y, Rankin G, Tu Y, Chen Y
    Int J Oncol, 2016;48(1):281-92.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Phospho-TOR (S2448) Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information