Detection of Human Phospho-TOR (S2448) by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival.
Mammalian Target of Rapamycin
Entrez Gene IDs:
2475 (Human); 287871 (Rat)
EC 220.127.116.11; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR
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