Human Phospho-TOR (S2448) Antibody

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Detection of Human Phospho-TOR (S2448) by Western Blot.
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Product Details
Citations (3)
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Human Phospho-TOR (S2448) Antibody Summary

Species Reactivity
Detects human Phospho-TOR (S2448) in ELISAs.
Monoclonal Rat IgG2A Clone # 834115
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing the human TOR S2448 site
Accession # P42345
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-TOR (S2448) antibody by Western Blot. View Larger

Detection of Human Phospho-TOR (S2448) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TOR

The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival.

Long Name
Mammalian Target of Rapamycin
Entrez Gene IDs
2475 (Human); 287871 (Rat)
Alternate Names
EC; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR

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Citations for Human Phospho-TOR (S2448) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis
    Authors: M Schilling, AB Prusty, B Boysen, FS Oppermann, YL Riedel, A Husedzinov, H Rasouli, A König, P Ramanathan, J Reymann, H Erfle, H Daub, U Fischer, OJ Gruss
    Cell Reports, 2021-06-22;35(12):109277.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC, Western Blot
  2. Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey
    Authors: MT Lane, TJ Herda, AC Fry, MA Cooper, MJ Andre, PM Gallagher
    Biol Sport, 2017-01-20;34(2):197-203.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Infared Microscopy, Western Blot
  3. Theaflavin-3, 3'-digallate decreases human ovarian carcinoma OVCAR-3 cell-induced angiogenesis via Akt and Notch-1 pathways, not via MAPK pathways.
    Authors: Gao Y, Rankin G, Tu Y, Chen Y
    Int J Oncol, 2015-11-20;48(1):281-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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