Human Piccolo Antibody Summary
Accession # NP_149015
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
C1qTNF3/CORS26 in Human Brain. C1qTNF3/CORS26 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human Piccolo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7935) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Piccolo (PCLO; also known as Aczonin) is a 530-570 kDa member of a small family of zinc finger proteins that are associated with the cytoskeletal matrix of exocytosis-competent cells. Its name (as well as that of oboe and bassoon) derives from the assumption that this molecule is part of an ensemble of "presynaptic" proteins that orchestrate secretory events at (presynaptic) cell membranes. Piccolo is known to be initially present in the trans-Golgi of neuronal cells. When needed, it is transported to the presynaptic density (or active zones) of GABAergic and glutamergic neurons, and to the plasma membrane of pancreatic beta -cells. It reportedly forms homodimers and heterodimers with Rim2, and serves as a Ca2+ sensor for exocytosis. Human Piccolo is 5065 amino acids (aa) in length. It is a multidomain protein with N-terminal Pro- and Gln-rich sequences, two C4-type Zn-finger motifs (aa 535-1028), one PZD domain (aa 4427-4478) and two membrane-binding C2 domains (aa 4565-5057). There are at least 30 potential Thr/Ser and two Tyr phosphorylation sites. Multiple splice variants exist that principally involve insertions and deletions. None of the splice forms are missing, or show substitutions for, the range of amino acids used by R&D Systems for antibody production. Two splice forms contain identical insertions of 7, 8, 9, 17, 43 and 54 aa, and differ only in a deletion of 60 aa at the C-terminus. A third splice variant shows a 48 aa substitution for aa 443-5065, while a fourth isoform utilizes an alternative start site at Met4459 that is coupled to 1) a 17 aa insertion after Lys4570, and 2) a deletion of aa 4798-5065. Over aa 4065-4328, human Piccolo shares 97% aa sequence identity with mouse Piccolo.
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