PINK1 in Human Brain. PINK1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Rat Anti-Human PINK1 Monoclonal Antibody (Catalog # MAB4357) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat‑induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti‑Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PTEN-induced putative kinase 1 (PINK1) is a ubiquitous protein with highest levels in heart, skeletal muscle, and testis, and with lower expression in other tissues, including brain. PINK1 contains a mitochondrial targeting motif and a highly conserved protein kinase domain with strong homology to serine/threonine kinases of the calcium/calmodulin family. Mutations in the Pink1 gene have been identified as a cause of autosomal recessive early-onset parkinsonism.
PTEN-induced Putative Kinase 1
Entrez Gene IDs:
65018 (Human); 68943 (Mouse); 298575 (Rat); 3925520 (T. castaneum)
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