Human PITX2 Antibody Summary
Accession # Q99697
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
PITX2 in Human Thyroid Cancer Tissue. PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive. Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.
Citation for Human PITX2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease
Authors: IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish
J. Neurosci., 2019;0(0):.
Sample Types: Whole Cells
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