Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Lizard - Gecko, Transgenic Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunocytochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PITX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PITX2
Ala149-Val317
Accession # Q99697

Specificity

Detects human PITX2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human PITX1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PITX2 Antibody

PITX2 antibody in Human Thyroid Cancer Tissue by Immunohistochemistry (IHC-P).

PITX2 in Human Thyroid Cancer Tissue.

PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PITX2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid cancer tissue

Reviewed Applications

Read 2 reviews rated 4.5 using AF7388 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PITX2

PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive.  Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.

Long Name

Pituitary Homeobox 2

Alternate Names

Brx1, IDG2, IGDS, IGDS2, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RP1, Solurshin

Entrez Gene IDs

5308 (Human); 18741 (Mouse); 54284 (Rat)

Gene Symbol

PITX2

UniProt

Q99697 (Human)

Additional PITX2 Products

Product Documents for Human PITX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PITX2 Antibody

For research use only

Citations for Human PITX2 Antibody

Customer Reviews for Human PITX2 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

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  • Human PITX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/03/2020
    Antibody was stained on E12.5 mouse heart sections (attached picture). Worked well. Antibody concentration used - 20ug/mL
    Human PITX2 Antibody AF7388
  • Human PITX2 Antibody
    Name: Zoe Crane-Smith
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 02/21/2018
    Used at 1:100, following citrate buffer antigen retrieval
    Human PITX2 Antibody AF7388

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human PITX2 Antibody

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  • Q: Does AF7388 recognize all 3 isoforms (a, b, and c) of Human PITX2?

    A: Yes, we would expect AF7388 to recognize all isoforms of Humaan PITX2 as the aa sequence used as the immunogen is present and unchanged in all three isoforms.

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