Human PITX2 Antibody

Catalog # Availability Size / Price Qty
PITX2 in Human Thyroid Cancer Tissue.
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Product Details
Citations (4)
Supplemental Products
Reviews (2)

Human PITX2 Antibody Summary

Species Reactivity
Detects human PITX2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human PITX1 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human PITX2
Accession # Q99697
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PITX2 antibody in Human Thyroid Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PITX2 in Human Thyroid Cancer Tissue. PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PITX2

PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive.  Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.

Long Name
Pituitary Homeobox 2
Entrez Gene IDs
5308 (Human); 18741 (Mouse); 54284 (Rat)
Alternate Names
all1-responsive gene 1; ALL1-responsive protein ARP1; ARP1MGC20144; Brx1; Homeobox protein PITX2; IDG2; IGDS; IGDS2; IHG2; IRID2; Otlx2; paired-like homeodomain 2; Paired-like homeodomain transcription factor 2MGC111022; pituitary homeo box 2; pituitary homeobox 2; PITX2; PTX2; RGS; RGSRIEG1Brx1; rieg bicoid-related homeobox transcription factor 1; RIEG bicoid-related homeobox transcription factor; RIEG; RIEG1; RP1; RS; Solurshin

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Citations for Human PITX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tooth Removal in the Leopard Gecko and the de novo Formation of Replacement Teeth
    Authors: KS Brink, JI Henríquez, TM Grieco, JR Martin Del, K Fu, JM Richman
    Frontiers in Physiology, 2021;12(0):576816.
    Species: Gecko
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cascade diversification directs generation of neuronal diversity in the hypothalamus
    Authors: YH Zhang, M Xu, X Shi, XL Sun, W Mu, H Wu, J Wang, S Li, P Su, L Gong, M He, M Yao, QF Wu
    Cell Stem Cell, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
    Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
    Cell Stem Cell, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease
    Authors: IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish
    J. Neurosci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Reviews for Human PITX2 Antibody

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Human PITX2 Antibody
By Anonymous on 12/03/2020
Application: ICC/IF Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Antibody was stained on E12.5 mouse heart sections (attached picture).
Worked well.
Antibody concentration used - 20ug/mL

Human PITX2 Antibody
By Zoe Crane-Smith on 02/21/2018
Application: ICC/IF Sample Tested: Brain tissue Species: Mouse

Used at 1:100, following citrate buffer antigen retrieval