Human PKC zeta Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PKC zeta in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PKC zeta
    Ile454-Val592
    Accession # Q05513
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PKC zeta by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, MCF‑7 human breast cancer cell line, and human lung tissue. PVDF Membrane was probed with 1 µg/mL of Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC zeta

PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.

  • Long Name:
    Protein Kinase C zeta
  • Entrez Gene IDs:
    5590 (Human); 18762 (Mouse); 25522 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.13; nPKC-zeta; PKC zeta; PKC2; PKC2PKC-ZETA; PRKCZ; protein kinase C zeta type; protein kinase C, zeta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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