The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 3 (Phospholipase C beta-3) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. The PLC-beta subfamily consists of 4 isozymes, beta 1‑4, which differ in their tissue distribution and their ability to be activated by G proteins. PLC-beta 1 and PLC-beta 3 are more widely expressed, whereas PLC-beta 2 is resticted to hematopoietic cells and PLC-beta 4 is limited to certain neuronal cells and the retina.
Human PLC‑ beta 3 Antibody
R&D Systems | Catalog # AF4716
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Lys27-Leu246
Accession # Q01970
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human PLC‑ beta 3 Antibody
Detection of Human PLC‑ beta 3 by Western Blot.
Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF mem-brane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approx-imately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PLC‑ beta 3 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human PLC‑ beta 3 Antibody
Western Blot
Sample: DU145 human prostate carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLC-beta 3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PLC-beta 3 Products
Product Documents for Human PLC‑ beta 3 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PLC‑ beta 3 Antibody
For research use only
Related Research Areas
Citations for Human PLC‑ beta 3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
