Human PLC‑ beta 3 Antibody Summary
Accession # Q01970
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PLC‑ beta 3 by Western Blot. Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF mem-brane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approx-imately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PLC‑ beta 3 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-beta 3
The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 3 (Phospholipase C beta-3) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. The PLC-beta subfamily consists of 4 isozymes, beta 1‑4, which differ in their tissue distribution and their ability to be activated by G proteins. PLC-beta 1 and PLC-beta 3 are more widely expressed, whereas PLC-beta 2 is resticted to hematopoietic cells and PLC-beta 4 is limited to certain neuronal cells and the retina.
Citations for Human PLC‑ beta 3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry
Authors: Y Hung, CC Chung, YC Chen, YH Kao, WS Lin, SA Chen, YJ Chen
Sample Types: Cell Lysates
Applications: Western Blot
M3 muscarinic receptor interaction with phospholipase C beta3 determines its signaling efficiency.
Authors: Kan W, Adjobo-Hermans M, Burroughs M, Faibis G, Malik S, Tall G, Smrcka A
J Biol Chem, 2014;289(16):11206-18.
Sample Types: Cell Lysates
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