Human PLZF Antibody

Catalog # Availability Size / Price Qty
AF2944
AF2944-SP
Detection of Human PLZF by Western Blot.
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Product Details
Citations (41)
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Human PLZF Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLZF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLZF
Met1-Gln254
Accession # Q05516
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PLZF antibody by Western Blot. View Larger

Detection of Human PLZF by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human PLZF antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human PLZF by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse PLZF by Western Blot View Larger

Detection of Mouse PLZF by Western Blot Increased mTORC1 activity in germ cells from Lkb1 newborn cKO testis. (a) Western blot of testis proteins at different postnatal ages using specific antibodies against p-S6K1 (T389), p-rpS6 (S235/6), rpS6 and beta -tubulin. rpS6, and beta -tubulin were used as internal controls. (b) Expression of PI3K-, mTOR- and SPC-related markers by western blot in P8 testes from control and Lkb1 cKO mice. (c) Immunohistochemistry for VASA, GATA4, p-rpS6 in P8 control testis. (d) Immunohistochemistry for Lin28a and p-rpS6 in P8 control testis. Serial sections were used in order to compare cellular localizations of these markers in the same tubule. Asterisks, different distribution of Lin28a and p-rpS6 in the same tubule resulting in strong positive for Lin28a and weak staining for p-rpS6. Arrows, cells expressing both Lin28a and p-rpS6. (e) Immunohistochemistry for Plzf and p-rpS6 in control and cKO mice. (f) Mean number of Plzf- or p-rpS6-positive cells in each tubule (positive cells/total tubules). The data were shown as mean±S.E.M. of at least three replicates. *P<0.05. Scale bars=50 μm Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse PLZF by Western Blot View Larger

Detection of Mouse PLZF by Western Blot Depletion of SPCs in Lkb1 cKO mice. Seminiferous tubules from 5- (a) and 13- (b) week-old control and Lkb1 cKO mice (n=3) were used for immunostaining using specific antibodies against PCNA, gamma H2AX and Plzf. Scale bars=50 μm. Arrows, representative Plzf-positive cells in tubules. (c) Quantification of Plzf-positive germ cells in each tubule. Data are presented as mean±S.E.M. **P < 0. 01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29022902), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name
Promyelocytic Leukemia Zinc Finger
Entrez Gene IDs
7704 (Human); 235320 (Mouse)
Alternate Names
PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein

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Citations for Human PLZF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice
    Authors: Peart NJ, Johnson TA, Lee S et al.
    PLOS Genetics
  2. Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation
    Authors: Yamanaka, S;Furihata, H;Yanagihara, Y;Taya, A;Nagasaka, T;Usui, M;Nagaoka, K;Shoya, Y;Nishino, K;Yoshida, S;Kosako, H;Tanokura, M;Miyakawa, T;Imai, Y;Shibata, N;Sawasaki, T;
    Nature communications
  3. Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells
    Authors: Hanna Valli, Meena Sukhwani, Serena L. Dovey, Karen A. Peters, Julia Donohue, Carlos A. Castro et al.
    Fertility and Sterility
  4. Gestational exposure to chlordecone promotes transgenerational changes in the murine reproductive system of males
    Authors: A Gely-Perno, C Hao, L Legoff, L Multigner, SC D'Cruz, C Kervarrec, B Jégou, S Tevosian, F Smagulova
    Sci Rep, 2018-07-06;8(1):10274.
  5. Centrosome linker protein C‐Nap1 maintains stem cells in mouse testes
    Authors: Hairuo Dang, Ana Martin‐Villalba, Elmar Schiebel
    EMBO reports
  6. Identification of dynamic undifferentiated cell states within the male germline
    Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
    Nat Commun, 2018-07-19;9(1):2819.
  7. LIN28A binds to meiotic gene transcripts and modulates translation in male germ cells
    Authors: M Wang, L Yu, S Wang, F Yang, M Wang, L Li, X Wu
    J. Cell. Sci., 2020-06-16;0(0):.
  8. Differential RA responsiveness among subsets of mouse late progenitor spermatogonia
    Authors: Shinnosuke Suzuki, John R. McCarrey, Brian P. Hermann
    Reproduction
  9. The regulatory repertoire of PLZF and SALL4 in undifferentiated spermatogonia
    Authors: DL Lovelace, Z Gao, K Mutoji, YC Song, J Ruan, BP Hermann
    Development, 2016-04-11;0(0):.
  10. GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance
    Authors: Hue M. La, Ai-Leen Chan, Julien M. D. Legrand, Fernando J. Rossello, Christina G. Gangemi, Antonella Papa et al.
    Development
  11. Establishment of a proteome profile and identification of molecular markers for mouse spermatogonial stem cells
    Authors: Quan Zhou, Yueshuai Guo, Bo Zheng, Binbin Shao, Min Jiang, Gaigai Wang et al.
    Journal of Cellular and Molecular Medicine
  12. SRSF1-mediated alternative splicing is required for spermatogenesis
    Authors: Lei, WL;Li, YY;Du, Z;Su, R;Meng, TG;Ning, Y;Hou, G;Schatten, H;Wang, ZB;Han, Z;Sun, F;Qian, WP;Liu, C;Sun, QY;
    International journal of biological sciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Divergent transcriptional regulation of astrocyte reactivity across disorders
    Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
    Nature, 2022-05-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Intraovarian, Isoform-Specific Transcriptional Roles of Progesterone Receptor in Ovulation
    Authors: KM Smith, DT Dinh, LK Akison, M Nicholls, KR Dunning, A Morimoto, JP Lydon, DL Russell, RL Robker
    Cells, 2022-05-05;11(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice
    Authors: V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand
    Stem Cell Research, 2022-02-25;60(0):102723.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues
    Authors: S Yamanaka, Y Horiuchi, S Matsuoka, K Kido, K Nishino, M Maeno, N Shibata, H Kosako, T Sawasaki
    Nature Communications, 2022-01-10;13(1):183.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
    Authors: WL Lei, YY Li, TG Meng, Y Ning, SM Sun, CH Zhang, Y Gui, ZB Wang, WP Qian, QY Sun
    Cell Death & Disease, 2021-09-27;12(10):883.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
    Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
    Elife, 2020-07-20;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Biological and RNA regulatory function of MOV10 in mammalian germ cells
    Authors: K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng
    BMC Biol., 2019-05-14;17(1):39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  20. PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
    Authors: RL Tang, LQ Fan
    Asian J. Androl., 2019-05-01;21(3):309-318.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia
    Authors: JMD Legrand, AL Chan, HM La, FJ Rossello, ML Änkö, FV Fuller-Pac, RM Hobbs
    Nat Commun, 2019;10(1):2278.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis
    Authors: SP Jamin, FG Petit, C Kervarrec, F Smagulova, D Illner, H Scherthan, M Primig
    Sci Rep, 2017-11-08;7(1):15065.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
    Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
    Biol. Reprod., 2017-03-01;96(3):707-719.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells
    Nat. Immunol, 2016-12-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Multiple layers of transcriptional regulation by PLZF in NKT-cell development
    Authors: Ai-Ping Mao
    Proc Natl Acad Sci USA, 2016-06-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  26. CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo.
    Authors: Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J
    PLoS ONE, 2015-11-24;10(11):e0143148.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. PAX7 expression defines germline stem cells in the adult testis.
    Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
    J Clin Invest, 2014-08-18;124(9):3929-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.
    Authors: Makela J, Toppari J, Rivero-Muller A, Ventela S
    PLoS ONE, 2014-03-11;9(3):e90088.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. PLZF mediates the PTEN/AKT/FOXO3a signaling in suppression of prostate tumorigenesis.
    Authors: Cao, JingPing, Zhu, Shu, Zhou, Wei, Li, Jie, Liu, Chang, Xuan, HanQing, Yan, Jie, Zheng, Lin, Zhou, LiXin, Yu, JianXiu, Chen, GuoQiang, Huang, YiRan, Yu, Zhuo, Feng, LiXin
    PLoS ONE, 2013-12-10;8(12):e77922.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  30. BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
    Authors: Mathew, Rebecca, Seiler, Michael, Scanlon, Seth T, Mao, Ai-ping, Constantinides, Michael, Bertozzi-Villa, Clara, Singer, Jeffrey, Bendelac, Albert
    Nature, 2012-10-21;491(7425):618-21.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  31. Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes
    Authors: Shun Bai, Kaiqiang Fu, Huiqi Yin, Yiqiang Cui, Qiuling Yue, Wenbo Li et al.
    Development
  32. Thalidomide and its metabolite 5‐hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF
    Authors: Satoshi Yamanaka, Hidetaka Murai, Daisuke Saito, Gembu Abe, Etsuko Tokunaga, Takahiro Iwasaki et al.
    The EMBO Journal
  33. Food-Derived High Arginine Peptides Promote Spermatogenesis Recovery in Busulfan Treated Mice
    Authors: Wenwen Liu, Lingfeng Zhang, Anning Gao, Muhammad Babar Khawar, Fengyi Gao, Wei Li
    Frontiers in Cell and Developmental Biology
  34. Granulocyte colony-stimulating factor (G-CSF) promotes spermatogenic regeneration from surviving spermatogonia after high-dose alkylating chemotherapy
    Authors: Travis Kotzur, Roberto Benavides-Garcia, Jennifer Mecklenburg, Jamila R. Sanchez, Matthew Reilly, Brian P. Hermann
    Reproductive Biology and Endocrinology
  35. Effects of chemotherapeutic agent bendamustine for non-hodgkin lymphoma on spermatogenesis in mice
    Authors: Min-Bo Minbo, Zhou Qiao, Yun-Fei Yunfei, Ming-Xi Mingxi, Zuo-Min Zuomin
    The Journal of Biomedical Research
  36. SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing
    Authors: Liu W, Lu X, Zhao ZH et al.
    eLife
  37. The epigenetic processes of meiosis in male mice are broadly affected by the widely used herbicide atrazine.
    Authors: Gely-Pernot A, Hao C, Becker E et al.
    BMC Genomics.
  38. PDCL2 is essential for spermiogenesis and male fertility in mice
    Authors: Minyan Li, Yuxi Chen, Jianping Ou, Junjiu Huang, Xiya Zhang
    Cell Death Discovery
  39. Retinoic Acid Receptors Control Spermatogonia Cell-Fate and Induce Expression of the SALL4A Transcription Factor
    Authors: Aurore Gely-Pernot, Mathilde Raverdeau, Marius Teletin, Nadège Vernet, Betty Féret, Muriel Klopfenstein et al.
    PLOS Genetics
  40. Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis
    Authors: S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime
    PLoS ONE, 2018-01-11;13(1):e0190925.
  41. SALL4 Expression in Gonocytes and Spermatogonial Clones of Postnatal Mouse Testes
    Authors: Kathrin Gassei, Kyle E. Orwig
    PLoS ONE

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